RRC ID 36300
Author Bader CA, Shandala T, Ng YS, Johnson IR, Brooks DA.
Title Atg9 is required for intraluminal vesicles in amphisomes and autolysosomes.
Journal Biol Open
Abstract Autophagy is an intracellular recycling and degradation process, which is important for energy metabolism, lipid metabolism, physiological stress response and organism development. During Drosophila development, autophagy is up-regulated in fat body and midgut cells, to control metabolic function and to enable tissue remodelling. Atg9 is the only transmembrane protein involved in the core autophagy machinery and is thought to have a role in autophagosome formation. During Drosophila development, Atg9 co-located with Atg8 autophagosomes, Rab11 endosomes and Lamp1 endosomes-lysosomes. RNAi silencing of Atg9 reduced both the number and the size of autophagosomes during development and caused morphological changes to amphisomes/autolysosomes. In control cells there was compartmentalised acidification corresponding to intraluminal Rab11/Lamp-1 vesicles, but in Atg9 depleted cells there were no intraluminal vesicles and the acidification was not compartmentalised. We concluded that Atg9 is required to form intraluminal vesicles and for localised acidification within amphisomes/autolysosomes, and consequently when depleted, reduced the capacity to degrade and remodel gut tissue during development.
Volume 4(11)
Pages 1345-55
Published 2015-9-9
DOI 10.1242/bio.013979
PII bio.013979
PMID 26353861
PMC PMC4728360
IF 2.029
Times Cited 20
Drosophila DGRC#112001