| RRC ID |
38453
|
| 著者 |
Ushida H, Kawakami T, Minami K, Chano T, Okabe H, Okada Y, Okamoto K.
|
| タイトル |
Methylation profile of DNA repetitive elements in human testicular germ cell tumor.
|
| ジャーナル |
Mol Carcinog
|
| Abstract |
Testicular germ cell tumors (TGCTs) have a unique epigenetic profile distinct from that of other types of cancer. To further evaluate epigenetics of TGCTs, this study examines DNA methylation patterns of DNA repetitive elements in TGCTs. Bisulfite genomic sequencing and combined bisulfite restriction analysis (COBRA) were used to analyze the methylation patterns of DNA repetitive elements (LINE1 and Alu repeats) in embryonal carcinoma (EC) derived cell lines, primary TGCT tissues, noncancerous testicular tissues adjacent to TGCTs and cancer cells derived from somatic tissues (testicular malignant lymphoma tissues and renal cell carcinoma cell lines). Through both bisulfite genomic sequencing and COBRA, LINE1 was extensively hypomethylated in both seminomatous and nonseminomatous TGCT tissues as well as EC cell lines. We studied two Alu repeats locating in the 5' end of E-cadherin and XIST by bisulfite genomic sequencing. These two Alu elements were extensively hypomethylated in seminomatous TGCTs, but methylated in nonseminomatous TGCTs, including two EC derived cell lines. This increased unmethylated profile in seminomatous TGCTs was observed also by COBRA for Alu repeats. Although partial demethylation of DNA repetitive elements was observed in cancer cells of somatic tissue origin, the degree of demethylation was more pronounced in TGCTs than in cancer cells of somatic tissue origin. We observed abnormal demethylation of DNA repetitive elements in some of the tissues adjacent to TGCTs. The results indicate that the underlying mechanisms to undergo or maintain demethylation of DNA repetitive sequences differ between TGCTs and cancer cells of somatic tissue origin.
|
| 巻・号 |
51(9)
|
| ページ |
711-22
|
| 公開日 |
2012-9-1
|
| DOI |
10.1002/mc.20831
|
| PMID |
21809391
|
| MeSH |
Adult
Aged
Biomarkers, Tumor / genetics*
Blotting, Western
Carcinoma, Embryonal / genetics
Carcinoma, Renal Cell / genetics
DNA (Cytosine-5-)-Methyltransferases / antagonists & inhibitors
DNA (Cytosine-5-)-Methyltransferases / genetics
DNA (Cytosine-5-)-Methyltransferases / metabolism
DNA Methylation*
DNA, Neoplasm / genetics
Epigenesis, Genetic
Gene Expression Profiling*
Humans
Kidney Neoplasms / genetics
Lymphoma / genetics*
Male
Middle Aged
Neoplasms, Germ Cell and Embryonal / genetics*
Polymerase Chain Reaction
RNA, Small Interfering / genetics
Repetitive Sequences, Nucleic Acid / genetics*
Testicular Neoplasms / genetics*
Testis / metabolism
Tumor Cells, Cultured
Young Adult
|
| IF |
3.825
|
| 引用数 |
31
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
