| RRC ID |
38569
|
| 著者 |
Yamamuro A, Kishino T, Ohshima Y, Yoshioka Y, Kimura T, Kasai A, Maeda S.
|
| タイトル |
Caspase-4 directly activates caspase-9 in endoplasmic reticulum stress-induced apoptosis in SH-SY5Y cells.
|
| ジャーナル |
J Pharmacol Sci
|
| Abstract |
The present study investigated the function of caspase-4 in endoplasmic reticulum (ER) stress-induced apoptosis in human neuronal cell line SH-SY5Y. Tunicamycin, which is known to induce ER stress, activated both caspase-9 and caspase-4, and the activation of caspase-4 preceded that of caspase-9. The caspase-4 inhibitor LEVD-CHO suppressed both the apoptosis and caspase-9 activation. In addition, human recombinant active caspase-4 cleaved wild type and D330A mutant substituted Asp-330 for alanine of human recombinant procaspase-9, but did not cleave D315A mutant substituted Asp-315 for alanine. These results suggest that caspase-4 directly activates caspase-9 by the processing of procaspase-9 at Asp-315 in ER stress-induced neuronal apoptosis.
|
| 巻・号 |
115(2)
|
| ページ |
239-43
|
| 公開日 |
2011-1-1
|
| DOI |
10.1254/jphs.10217sc
|
| PII |
JST.JSTAGE/jphs/10217SC
|
| PMID |
21282934
|
| MeSH |
Apoptosis / drug effects
Caspase 9 / metabolism*
Caspase Inhibitors
Caspases, Initiator / metabolism*
Cell Line
Endoplasmic Reticulum / drug effects
Endoplasmic Reticulum / physiology*
Enzyme Activation
Humans
Neurons / cytology
Neurons / enzymology
Neurons / physiology*
Recombinant Proteins / metabolism
Stress, Physiological*
Tunicamycin / pharmacology*
|
| IF |
2.835
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
|
