RRC ID 38668
Author Saga Y, Yagi T, Ikawa Y, Sakakura T, Aizawa S.
Title Mice develop normally without tenascin.
Journal Genes Dev
Abstract Tenascin, an extracellular matrix protein, is expressed in an unusually restricted pattern during embryogenesis and has been implicated in a variety of morphogenetic phenomena. To directly assess the function of tenascin in vivo, we generated mutant mice in which the tenascin gene was nully disrupted by replacing it with the lacZ gene. In mutant mice, lacZ was expressed in place of tenascin, and no tenascin product was detected. Homozygous mutant mice were, however, obtained in accordance with Mendelian laws, and both females and males produced offspring normally. No anatomical or histological abnormalities were detected in any tissues, and no major changes were observed in distribution of fibronectin, laminin, collagen, and proteoglycan. The existence of these mutant mice, lacking tenascin yet phenotypically normal, casts doubt on the theory that tenascin plays and essential role in normal development.
Volume 6(10)
Pages 1821-31
Published 1992-10
DOI 10.1101/gad.6.10.1821
PMID 1383086
MeSH Animals Base Sequence Blotting, Northern Blotting, Southern Cell Adhesion Molecules, Neuronal / genetics Cell Adhesion Molecules, Neuronal / physiology* Cells, Cultured Chimera Embryonic and Fetal Development / genetics Embryonic and Fetal Development / physiology* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / physiology* Female Immunohistochemistry Lac Operon Male Mice Mice, Inbred C57BL Mice, Inbred CBA Mice, Mutant Strains Molecular Sequence Data Mutagenesis Oligonucleotides Polymerase Chain Reaction Tenascin Transfection
IF 8.99
Times Cited 531
Human and Animal Cells