RRC ID 38942
Author Yamauchi Y, Kobayashi M, Tanaka A.
Title Application of a dicistronic mRNA expression vector to antisense RNA expression in mammalian cells.
Journal Antisense Nucleic Acid Drug Dev
Abstract One important role of antisense RNA expression in mammalian cell biology is to partially suppress the functions of target genes of which the functions are unknown but which are indispensable for host cell growth. Therefore, we applied a dicistronic mRNA expression vector to express antisense p53 RNA in mouse fibrosarcoma MethA cells. We also established several clones in which the p53 gene functions were partially suppressed by the introduced antisense RNA. Compared with the control MethA clones, the contents of p53 protein in those expressing the antisense RNA were about halved, and their growth rates were remarkably decreased. These results proved that an antisense RNA in a dicistronic mRNA construct decreases the cellular content of a targeted gene product and that clones harboring the dicistronic mRNA construct can be established in which expression levels of the introduced antisense RNA are controlled by the stringency of drug selection.
Volume 6(4)
Pages 305-9
Published 1996-1-1
DOI 10.1089/oli.1.1996.6.305
PMID 9012867
MeSH Animals Clone Cells Genes* Genetic Vectors* Mice Mice, Inbred BALB C Mutation Plasmids / genetics RNA, Antisense / biosynthesis* Tumor Cells, Cultured Tumor Suppressor Protein p53 / genetics*
Times Cited 3
WOS Category MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells