RRC ID 39277
著者 Takeda K, Ichijo H, Fujii M, Mochida Y, Saitoh M, Nishitoh H, Sampath TK, Miyazono K.
タイトル Identification of a novel bone morphogenetic protein-responsive gene that may function as a noncoding RNA.
ジャーナル J Biol Chem
Abstract Bone morphogenetic proteins (BMPs)/osteogenic proteins (OPs), members of the transforming growth factor-beta superfamily, have a wide variety of effects on many cell types including osteoblasts and chondroblasts, and play critical roles in embryonic development. BMPs transduce their effects through binding to two different types of serine/threonine kinase receptors, type I and type II. Signaling by these receptors is mediated by the recently identified Smad proteins. Despite the rapid progress in understanding of the signaling mechanism downstream of BMP receptors, the target genes of BMPs are poorly understood in mammals. Here we identified a novel gene, termed BMP/OP-responsive gene (BORG), in C2C12 mouse myoblast cell line which trans-differentiates into osteoblastic cells in response to BMPs. Expression of BORG was dramatically induced in C2C12 cells by the treatment with BMP-2 or OP-1 within 2 h and peaked at 12-24 h, whereas transforming growth factor-beta had a minimal effect. BMP-dependent expression of BORG was also detected in other cell types which are known to respond to BMPs, suggesting that BORG is a common target gene of BMPs. Cloning and sequence analysis of BORG cDNA and the genomic clones revealed that, unexpectedly, the transcript of BORG lacks any extensive open reading frames and contains a cluster of multiple interspersed repetitive sequences in its middle part. The unusual structural features suggested that BORG may function as a noncoding RNA, although it is spliced and polyadenylated as authentic protein-coding mRNAs. Together with the observation that transfection of antisense oligonucleotides of BORG partially inhibited BMP-induced differentiation in C2C12 cells, it is possible that a new class of RNA molecules may have certain roles in the differentiation process induced by BMPs.
巻・号 273(27)
ページ 17079-85
公開日 1998-7-3
DOI 10.1074/jbc.273.27.17079
PII S0021-9258(18)80647-0
PMID 9642273
MeSH Animals Base Sequence Blotting, Northern Bone Morphogenetic Proteins / metabolism* Cell Line Cloning, Molecular DNA, Complementary Exons Introns Mice Mice, Inbred C3H Molecular Sequence Data Oligonucleotides, Antisense / pharmacology RNA / genetics RNA / metabolism*
IF 4.238
引用数 42
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 ST2(RCB0224)