RRC ID 39410
著者 Yukawa O, Nakajima T, Yukawa M, Ozawa T, Yamada T.
タイトル Induction of radical scavenging ability and protection against radiation-induced damage to microsomal membranes following low-dose irradiation.
ジャーナル Int J Radiat Biol
Abstract PURPOSE:To investigate changes in rat liver cytosolic radical scavenging ability and in microsomal membrane function following low doses of radiation.
MATERIALS AND METHODS:Wistar rats were irradiated with 1-50 cGy of X-rays and liver cytosolic radical scavenging ability was determined using DPPH, a stable free radical. Liver microsomal drug metabolizing activity was determined using hexobarbital as a substrate. Cytosolic antioxidants and microsomal enzymes were spectrophotometrically determined.
RESULTS:Irradiation of rats at around 5-10 cGy induced liver cytosolic radical scavenging ability and a considerable increase in this ability at 5 cGy was observed for 3 days after irradiation. Glutathione reductase was suggested as a candidate of cytosolic antioxidants. Radiation-induced damage to liver microsomal drug metabolizing enzyme activity was suppressed by preirradiation with 5 cGy, mainly by protecting cytochrome P-450.
CONCLUSIONS:Low doses of radiation increased cytosolic radical scavenging ability and resulted in protection of microsomal membrane function which is easily damaged by radiation-induced free radicals.
巻・号 75(9)
ページ 1189-99
公開日 1999-9-1
DOI 10.1080/095530099139665
PMID 10528927
MeSH Animals Cell Membrane / radiation effects Cells, Cultured Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System / metabolism Dose-Response Relationship, Radiation Male Microsomes, Liver / enzymology Microsomes, Liver / radiation effects* NADP / metabolism Radiation Protection* Rats Rats, Wistar Reactive Oxygen Species Superoxide Dismutase / metabolism
IF 2.368
引用数 21
WOS 分野 NUCLEAR SCIENCE & TECHNOLOGY RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING BIOLOGY
リソース情報
ヒト・動物細胞