論文 - 詳細
RRC ID | 39410 |
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著者 | Yukawa O, Nakajima T, Yukawa M, Ozawa T, Yamada T. |
タイトル | Induction of radical scavenging ability and protection against radiation-induced damage to microsomal membranes following low-dose irradiation. |
ジャーナル | Int J Radiat Biol |
Abstract |
PURPOSE:To investigate changes in rat liver cytosolic radical scavenging ability and in microsomal membrane function following low doses of radiation. MATERIALS AND METHODS:Wistar rats were irradiated with 1-50 cGy of X-rays and liver cytosolic radical scavenging ability was determined using DPPH, a stable free radical. Liver microsomal drug metabolizing activity was determined using hexobarbital as a substrate. Cytosolic antioxidants and microsomal enzymes were spectrophotometrically determined. RESULTS:Irradiation of rats at around 5-10 cGy induced liver cytosolic radical scavenging ability and a considerable increase in this ability at 5 cGy was observed for 3 days after irradiation. Glutathione reductase was suggested as a candidate of cytosolic antioxidants. Radiation-induced damage to liver microsomal drug metabolizing enzyme activity was suppressed by preirradiation with 5 cGy, mainly by protecting cytochrome P-450. CONCLUSIONS:Low doses of radiation increased cytosolic radical scavenging ability and resulted in protection of microsomal membrane function which is easily damaged by radiation-induced free radicals. |
巻・号 | 75(9) |
ページ | 1189-99 |
公開日 | 1999-9-1 |
DOI | 10.1080/095530099139665 |
PMID | 10528927 |
MeSH | Animals Cell Membrane / radiation effects Cells, Cultured Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System / metabolism Dose-Response Relationship, Radiation Male Microsomes, Liver / enzymology Microsomes, Liver / radiation effects* NADP / metabolism Radiation Protection* Rats Rats, Wistar Reactive Oxygen Species Superoxide Dismutase / metabolism |
IF | 2.368 |
引用数 | 21 |
WOS 分野 | NUCLEAR SCIENCE & TECHNOLOGY RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING BIOLOGY |
リソース情報 | |
ヒト・動物細胞 |