RRC ID |
39477
|
著者 |
Kuboki T, Hattori T, Mizushima T, Kanyama M, Fujisawa T, Yamashita A, Takigawa M.
|
タイトル |
Detection of specific antibodies against human cultured chondrosarcoma (HCS-2/8) and osteosarcoma (Saos-2) cells in the serum of patients with osteoarthritis of the temporomandibular joint.
|
ジャーナル |
Arch Oral Biol
|
Abstract |
To find specific humoral antibodies in sera from patients with temporomandibular joint (TMJ) osteoarthritis (OA), an immortal human chondrocyte (HCS-2/8) and osteoblast (Saos-2) cell line derived from a chondrosarcoma and an osteosarcoma, respectively, were used as source proteins of human antigens. Patients with chronically painful TMJ OA (n = 18) but no other joints symptoms were selected from a consecutive series of patients with temperomandibular disorders and sex-matched asymptomatic controls (n = 8) were also recruited. Cellular proteins of the HCS-2/8 and Saos-2 cells were subjected to Western blotting with the OA and control sera as probes. Band-recognition frequency and the peak optical density of the band were compared between groups by chi2 and t-tests. OA sera recognized various bands for the chondrocytes, and one of these (47-kDa) was specific for the OA sera. In two OA patients whose treatment outcome was less favorable, the reactivity against the 47-kDa protein was relatively high. In addition, the OA sera clearly cross-reacted with recombinant HSP47. Based on these findings, an autoimmune reaction against chondrocytes could be one of the exaggerating and/or perpetuating mechanisms in the pathophysiology of osteoarthritic TMJs, and the humoral antibody titre against the HSP47-like protein derived from the chondrocytes could be one of the possible markers for the prognosis of the joint pathology.
|
巻・号 |
44(5)
|
ページ |
403-14
|
公開日 |
1999-5-1
|
DOI |
10.1016/s0003-9969(99)00009-6
|
PII |
S0003-9969(99)00009-6
|
PMID |
10391498
|
MeSH |
Adolescent
Adult
Aged
Antibodies, Neoplasm / blood*
Autoantibodies / immunology
Biomarkers / analysis
Blotting, Western
Case-Control Studies
Chi-Square Distribution
Chondrocytes / immunology
Chondrosarcoma / immunology*
Cross Reactions
Female
HSP47 Heat-Shock Proteins
Heat-Shock Proteins / immunology
Humans
Male
Middle Aged
Osteoarthritis / blood
Osteoarthritis / immunology*
Osteoblasts / immunology
Osteosarcoma / immunology*
Prognosis
Recombinant Proteins
Temporomandibular Joint Disorders / blood
Temporomandibular Joint Disorders / immunology*
Treatment Outcome
Tumor Cells, Cultured
|
IF |
1.931
|
引用数 |
5
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
リソース情報 |
ヒト・動物細胞 |
|