| RRC ID |
39556
|
| 著者 |
Yamada S, Park SY, Shimizu H, Koshizuka Y, Kadokura K, Satoh T, Suruga K, Ogawa M, Isogai Y, Nishio T, Shiro Y, Oku T.
|
| タイトル |
Structure of cytochrome c6 from the red alga Porphyra yezoensis at 1. 57 A resolution.
|
| ジャーナル |
Acta Crystallogr D Biol Crystallogr
|
| Abstract |
The crystal structure of cytochrome c(6) from the red alga Porphyra yezoensis has been determined at 1.57 A resolution. The crystal is tetragonal and belongs to space group P4(3)2(1)2, with unit-cell parameters a = b = 49.26 (3), c = 83.45 (4) A and one molecule per asymmetric unit. The structure was solved by the molecular-replacement method and refined with X-PLOR to an R factor of 19.9% and a free R factor of 25.4%. The overall structure of cytochrome c(6) follows the topology of class I c-type cytochromes in which the heme prosthetic group covalently binds to Cys14 and Cys17, and the iron has an octahedral coordination with His18 and Met58 as the axial ligands. The sequence and the structure of the eukaryotic red algal cytochrome c(6) are very similar to those of a prokaryotic cyanobacterial cytochrome c(6) rather than those of eukaryotic green algal c(6) cytochromes.
|
| 巻・号 |
56(Pt 12)
|
| ページ |
1577-82
|
| 公開日 |
2000-12-1
|
| DOI |
10.1107/s090744490001461x
|
| PII |
S090744490001461X
|
| PMID |
11092924
|
| MeSH |
Amino Acid Sequence
Chlorophyta / chemistry
Crystallography, X-Ray
Cytochromes / chemistry*
Cytochromes / isolation & purification
Cytochromes f
Heme / chemistry
Models, Molecular
Molecular Sequence Data
Protein Conformation
Rhodophyta / chemistry*
Sequence Homology, Amino Acid
|
| IF |
5.266
|
| 引用数 |
28
|
|
WOS 分野
|
CRYSTALLOGRAPHY
BIOCHEMICAL RESEARCH METHODS
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
