RRC ID 39757
著者 Okada Y, Okada T, Numata M, Hayashi Y, Yamashima T, Yamashita J.
タイトル Increased expression of deoxyribonucleic acid methyltransferase gene in human astrocytic tumors.
ジャーナル Neurol Med Chir (Tokyo)
Abstract The relationship between the grade of astrocytic tumor and the expression of deoxyribonucleic acid methyltransferase (DNA-MTase) gene was examined. The levels of DNA-MTase messenger ribonucleic acid (mRNA) were measured by semiquantitative reverse transcriptase-polymerase chain reaction in surgical specimens from 12 astrocytic tumors (4 astrocytomas, 6 anaplastic astrocytomas, and 2 glioblastomas) and two normal brain tissues, and in four glioma cell lines. Compared to normal brain tissues, the levels of DNA-MTase mRNA were increased by 16- to 55-fold in low grade astrocytomas, and significantly increased by 200- to 4500-fold in high grade astrocytomas (anaplastic astrocytomas and glioblastomas) and more than 4500-fold in glioma cell lines. In situ hybridization with paraffin-embedded surgical specimens of human astrocytic tumors showed DNA-MTase mRNA was abundantly expressed in high grade astrocytomas. The detection of increased DNA-MTase expression in astrocytic tumor indicates involvement in the tumorigenesis and suggests that blocking of this change with specific inhibitors may offer new therapeutic strategies for malignant astrocytic tumors.
巻・号 40(11)
ページ 564-70; discussion 570-1
公開日 2000-11-1
DOI 10.2176/nmc.40.564
PII JST.JSTAGE/nmc/40.564
PMID 11109793
MeSH Astrocytoma / genetics* Blotting, Southern Brain Neoplasms / genetics* DNA, Neoplasm / genetics* Gene Expression Regulation, Neoplastic Glioblastoma / genetics Humans In Situ Hybridization In Vitro Techniques Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured tRNA Methyltransferases / genetics*
IF 1.836
引用数 0
WOS 分野 CLINICAL NEUROLOGY SURGERY
リソース情報
ヒト・動物細胞