| RRC ID |
40803
|
| 著者 |
Phongsopitanun W, Tanasupawat S, Suwanborirux K, Ohkuma M, Kudo T.
|
| タイトル |
Actinomadura rayongensis sp. nov., isolated from peat swamp forest soil.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel actinomycete strain RY35-68(T), isolated from a peat swamp forest soil sample in Rayong Province, Thailand, was characterized using a polyphasic approach. The strain belonged to the genus Actinomadura based on morphological and chemotaxonomic characteristics. Cell-wall analysis revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan layer. The diagnostic sugar in whole-cell hydrolysates was identified as madurose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The major cellular fatty acids were C16 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The genomic DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence similarity analysis, strain RY35-68(T) was closely related to the species Actinomadura atramentaria JCM 6250(T) (97.5 %). The value of DNA-DNA relatedness between strain RY35-68(T) and A. atramentaria JCM 6250(T) was 37.6-42.6 %. On the basis of its phenotypic characteristics and these results mentioned, this strain could be distinguished from the closely related type strain and represents a novel species of the genus Actinomadura, for which the name Actinomadura rayongensis sp. nov. (type strain RY35-68(T) = JCM 19830(T) = TISTR 2211(T) = PCU 332(T)) is proposed.
|
| 巻・号 |
65(Pt 3)
|
| ページ |
890-895
|
| 公開日 |
2015-3-1
|
| DOI |
10.1099/ijs.0.000033
|
| PMID |
25525125
|
| MeSH |
Actinomycetales / classification*
Actinomycetales / genetics
Actinomycetales / isolation & purification
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Diaminopimelic Acid / chemistry
Fatty Acids / chemistry
Forests
Molecular Sequence Data
Nucleic Acid Hybridization
Peptidoglycan / chemistry
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Thailand
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
Wetlands*
|
| IF |
2.415
|
| 引用数 |
5
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 6250
JCM 19830 |
