RRC ID 41025
著者 Park S, Park JM, Sun Joo E, Won SM, Kyum Kim M, Yoon JH.
タイトル Sphingomicrobium aestuariivivum sp. nov., isolated from a tidal flat.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, designated AH-M8T, was isolated from a tidal flat sediment collected from Aphae Island in the south-western sea, South Korea. Strain AH-M8T grew optimally at 35 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain AH-M8T belonged to the genus Sphingomicrobium, clustering with the type strain of Sphingomicrobium astaxanthinifaciens, with which it shared 99.0% 16S rRNA gene sequence similarity. Sequence similarities to the type strains of other species of the genus Sphingomicrobium were 95.4-96.0%. Strain AH-M8T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as the major fatty acids. The major polar lipids of strain AH-M8T were phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and one unidentified glycolipid. The major polyamine is triamine sym-homospermidine. The DNA G+C content of strain AH-M8T was 66.7 mol% and its mean DNA-DNA relatedness value with S. astaxanthinifaciens JCM 18551T was 21%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain AH-M8T is separated from other species of the genus Sphingomicrobium. On the basis of the data presented, strain AH-M8T is considered to represent a novel species of the genus Sphingomicrobium, for which the name Sphingomicrobium aestuariivivum sp. nov. is proposed. The type strain is AH-M8T ( = KCTC 42286T = NBRC 110678T).
巻・号 65(8)
ページ 2678-2683
公開日 2015-8-1
DOI 10.1099/ijs.0.000320
PMID 25964515
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Geologic Sediments / microbiology* Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Republic of Korea Seawater / microbiology* Sequence Analysis, DNA Spermidine / analogs & derivatives Spermidine / chemistry Sphingomonadaceae / classification* Sphingomonadaceae / genetics Sphingomonadaceae / isolation & purification Ubiquinone / chemistry
IF 2.415
引用数 2
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 18551