RRC ID 41040
著者 Zhong ZP, Liu Y, Hou TT, Liu HC, Zhou YG, Wang F, Liu ZP.
タイトル Pseudomonas salina sp. nov., isolated from a salt lake.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-staining-negative, facultatively aerobic bacterium, strain XCD-X85(T), was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9%, w/v) in Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X85(T) were non-endospore-forming rods, 0.4-0.6 μm wide and 1.0-1.6 μm long, and motile by means of a single polar flagellum. Strain XCD-X85(T) was catalase- and oxidase-positive. Growth was observed in the presence of 0-12.0% (w/v) NaCl (optimum, 1.0-2.0%) and at 4-35 °C (optimum, 25-30 °C) and pH 6.5-10.5 (optimum, pH 8.0-8.5). Strain XCD-X85(T) contained (>10%) summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C12 : 0, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the predominant fatty acids. The major respiratory quinone was ubiquinone 9 (Q-9). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 57.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X85(T) was associated with the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to Pseudomonas pelagia CL-AP6(T) (99.0%) and Pseudomonas bauzanensis BZ93(T) (96.8%). DNA-DNA relatedness of strain XCD-X85T to P. pelagia JCM 15562(T) was 19 ± 1%. On the basis of the data presented above, it is concluded that strain XCD-X85(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salina sp. nov. is proposed. The type strain is XCD-X85(T) ( = CGMCC 1.12482(T) = JCM 19469(T)).
巻・号 65(9)
ページ 2846-2851
公開日 2015-9-1
DOI 10.1099/ijs.0.000341
PMID 25985833
MeSH Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Lakes / microbiology* Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* Pseudomonas / classification* Pseudomonas / genetics Pseudomonas / isolation & purification RNA, Ribosomal, 16S / genetics Salinity* Sequence Analysis, DNA Ubiquinone / chemistry Water Microbiology*
IF 2.415
引用数 12
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 15562 JCM 19469