RRC ID 41569
著者 Hatano H, Sarkar G, Bolander ME.
タイトル Development of a cellular model to study alternative splicing of type II collagen gene.
ジャーナル J Orthop Res
Abstract To study regulation of alternative splicing of type II collagen (COL2) pre-mRNA, we constructed a mouse COL2 "minigene" containing genomic sequences spanning exon 1 to exon 4 of COL2 downstream of a cytomegalovirus (CMV) promoter. This minigene was introduced into ATDC5 cells, which undergo chondrocytic differentiation when treated with insulin. Alternative splicing of the COL2 minigene was evaluated by comparing the expression of the two mRNAs transcribed from the minigene to the expression of alternatively spliced transcripts from the endogenous COL2 gene. This analysis suggested that regulation of alternative splicing of pre-mRNAs from the minigene and the endogenous COL2 gene are accomplished by similar mechanisms. We conclude that the cloned genomic fragment contains key sequences necessary for alternative splicing of COL2 pre-mRNA. This system provides a useful model to begin the process of identifying cis- and trans-acting factors that carry out alternative splicing of COL2 pre-mRNA during chondrocyte differentiation.
巻・号 20(3)
ページ 516-9
公開日 2002-5-1
DOI 10.1016/S0736-0266(01)00123-1
PMID 12038625
MeSH Alternative Splicing* Animals Cell Line Collagen Type II / genetics* Cytomegalovirus / genetics Mice Peptide Fragments / genetics Promoter Regions, Genetic / genetics RNA Precursors / genetics RNA, Messenger / metabolism
IF 2.728
引用数 8
WOS 分野 ORTHOPEDICS
リソース情報
ヒト・動物細胞