論文 - 詳細
| RRC ID | 4223 |
|---|---|
| 著者 | Doitsidou M, Flames N, Lee AC, Boyanov A, Hobert O. |
| タイトル | Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans. |
| ジャーナル | Nat Methods |
| Abstract |
We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately. |
| 巻・号 | 5(10) |
| ページ | 869-72 |
| 公開日 | 2008-10-1 |
| DOI | 10.1038/nmeth.1250 |
| PII | nmeth.1250 |
| PMID | 18758453 |
| PMC | PMC2693092 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Dopamine / metabolism* Flow Cytometry Fluorescence Green Fluorescent Proteins / genetics Mutation* Neurons / metabolism* |
| IF | 30.822 |
| 引用数 | 55 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| リソース情報 | |
| 線虫 | tm2044 tm1446 |