RRC ID 42594
Author Takahashi N, Kitazawa H, Shimosato T, Iwabuchi N, Xiao JZ, Iwatsuki K, Kokubo S, Saito T.
Title An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro.
Journal FEMS Immunol Med Microbiol
Abstract The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.
Volume 46(3)
Pages 461-9
Published 2006-4-1
DOI 10.1111/j.1574-695X.2006.00064.x
PMID 16553822
MeSH Animals B-Lymphocytes / cytology B-Lymphocytes / immunology Base Sequence Bifidobacterium / genetics* Bifidobacterium / immunology* Cell Proliferation DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Bacterial / immunology* Enzyme-Linked Immunosorbent Assay Immunoglobulin E / biosynthesis* Immunoglobulin E / immunology Immunoglobulin Isotypes / immunology Interferon-gamma / immunology Interleukin-12 / immunology Interleukin-4 / immunology Mice Mice, Inbred BALB C Molecular Sequence Data NF-kappa B / immunology Oligodeoxyribonucleotides / genetics* Oligodeoxyribonucleotides / immunology Oligodeoxyribonucleotides / isolation & purification Probiotics / pharmacology* Sequence Analysis, DNA Specific Pathogen-Free Organisms
Times Cited 34
Human and Animal Cells J774.1