| RRC ID |
42594
|
| Author |
Takahashi N, Kitazawa H, Shimosato T, Iwabuchi N, Xiao JZ, Iwatsuki K, Kokubo S, Saito T.
|
| Title |
An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro.
|
| Journal |
FEMS Immunol Med Microbiol
|
| Abstract |
The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.
|
| Volume |
46(3)
|
| Pages |
461-9
|
| Published |
2006-4-1
|
| DOI |
10.1111/j.1574-695X.2006.00064.x
|
| PII |
FIM064
|
| PMID |
16553822
|
| MeSH |
Animals
B-Lymphocytes / cytology
B-Lymphocytes / immunology
Base Sequence
Bifidobacterium / genetics*
Bifidobacterium / immunology*
Cell Proliferation
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Bacterial / immunology*
Enzyme-Linked Immunosorbent Assay
Immunoglobulin E / biosynthesis*
Immunoglobulin E / immunology
Immunoglobulin Isotypes / immunology
Interferon-gamma / immunology
Interleukin-12 / immunology
Interleukin-4 / immunology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
NF-kappa B / immunology
Oligodeoxyribonucleotides / genetics*
Oligodeoxyribonucleotides / immunology
Oligodeoxyribonucleotides / isolation & purification
Probiotics / pharmacology*
Sequence Analysis, DNA
Specific Pathogen-Free Organisms
|
| Times Cited |
34
|
|
WOS Category
|
INFECTIOUS DISEASES
IMMUNOLOGY
MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
J774.1 |
