| RRC ID |
4274
|
| 著者 |
Ow MC, Martinez NJ, Olsen PH, Silverman HS, Barrasa MI, Conradt B, Walhout AJ, Ambros V.
|
| タイトル |
The FLYWCH transcription factors FLH-1, FLH-2, and FLH-3 repress embryonic expression of microRNA genes in C. elegans.
|
| ジャーナル |
Genes Dev
|
| Abstract |
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression post-transcriptionally via antisense base-pairing. Although miRNAs are involved in a variety of important biological functions, little is known about their transcriptional regulation. Using yeast one-hybrid assays, we identified transcription factors with a FLYWCH Zn-finger DNA-binding domain that bind to the promoters of several Caenorhabditis elegans miRNA genes. The products of the flh-1 and flh-2 genes function redundantly to repress embryonic expression of lin-4, mir-48, and mir-241, miRNA genes that are normally expressed only post-embryonically. Although single mutations in either flh-1 or flh-2 genes result in a viable phenotype, double mutation of flh-1 and flh-2 results in early larval lethality and an enhanced derepression of their target miRNAs in embryos. Double mutations in flh-2 and a third FLYWCH Zn-finger-containing transcription factor, flh-3, also result in enhanced precocious expression of target miRNAs. Mutations of lin-4 or mir-48&mir-241 do not rescue the lethal flh-1; flh-2 double-mutant phenotype, suggesting that the inviability is not solely the result of precocious expression of these miRNAs. Therefore, the FLH-1 and FLH-2 proteins likely play a more general role in regulating gene expression in embryos.
|
| 巻・号 |
22(18)
|
| ページ |
2520-34
|
| 公開日 |
2008-9-15
|
| DOI |
10.1101/gad.1678808
|
| PII |
22/18/2520
|
| PMID |
18794349
|
| PMC |
PMC2546698
|
| MeSH |
Amino Acid Sequence
Animals
Binding Sites
Caenorhabditis elegans / embryology
Caenorhabditis elegans / genetics*
Gene Expression Regulation, Developmental*
Genes, Helminth*
MicroRNAs / genetics*
Molecular Sequence Data
RNA Interference
Sequence Homology, Amino Acid
Transcription Factors / chemistry
Transcription Factors / metabolism
Transcription Factors / physiology*
|
| IF |
9.527
|
| 引用数 |
33
|
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2118
tm2126
tm3024 |
