RRC ID 42765
著者 Sawada R, Ito T, Tsuchiya T.
タイトル Changes in expression of genes related to cell proliferation in human mesenchymal stem cells during in vitro culture in comparison with cancer cells.
ジャーナル J Artif Organs
Abstract We investigated the expression levels of several genes related to cell proliferation in human mesenchymal stem cells (hMSCs) during in vitro culture for use in clinical applications. In this study, we focused on the relationship between hMSC proliferation and transforming growth factor beta (TGFbeta) signaling during in vitro culture. The proliferation rate of hMSCs gradually decreased and marked changes in hMSC morphology were not observed in 3 months of in vitro culture. The mRNA expressions of TGFbeta1, TGFbeta2, and TGFbeta receptor type I (TGFbetaRI) in hMSCs increased with the length of cell culture. There had been no change in the TGFbeta3, TGFbetaRII, and TGFbetaRIII mRNA expressions by the 12th passage from the primary culture (at about 3 months). The mRNA expression of Smad3 increased, but those of c-myc and nucleostemin decreased with the length of hMSC in vitro culture. In addition, the expression profiles of the genes that regulate cellular proliferation in hMSCs were significantly different from those of cancer cells. In conclusion, hMSCs derived from bone marrow seldom underwent spontaneous transformation during 1-2 months of in vitro culture for use in clinical applications. In hMSCs as well as in epithelial cells, growth might be controlled by the TGFbeta family signaling.
巻・号 9(3)
ページ 179-84
公開日 2006-1-1
DOI 10.1007/s10047-006-0338-z
PMID 16998703
MeSH Activin Receptors, Type I / genetics* Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / pathology Cell Proliferation* Follow-Up Studies Gene Expression* HeLa Cells / pathology Humans Liver Neoplasms / genetics Liver Neoplasms / pathology* Mesenchymal Stem Cells / pathology* Protein Serine-Threonine Kinases RNA, Messenger / genetics* Receptor, Transforming Growth Factor-beta Type I Receptors, Transforming Growth Factor beta / genetics* Reverse Transcriptase Polymerase Chain Reaction Transforming Growth Factor beta / genetics* Transforming Growth Factor beta1 / genetics Transforming Growth Factor beta2 / genetics Tumor Cells, Cultured
IF 1.223
リソース情報
ヒト・動物細胞