| RRC ID |
43251
|
| 著者 |
Mizuno Y, Yagi K, Tokuzawa Y, Kanesaki-Yatsuka Y, Suda T, Katagiri T, Fukuda T, Maruyama M, Okuda A, Amemiya T, Kondoh Y, Tashiro H, Okazaki Y.
|
| タイトル |
miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.
|
| 巻・号 |
368(2)
|
| ページ |
267-72
|
| 公開日 |
2008-4-4
|
| DOI |
10.1016/j.bbrc.2008.01.073
|
| PII |
S0006-291X(08)00095-8
|
| PMID |
18230348
|
| MeSH |
Animals
Cell Differentiation
Cell Line
Cell Proliferation
Down-Regulation
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism*
Mice
MicroRNAs / metabolism*
Osteoblasts / cytology*
Osteoblasts / metabolism*
Osteogenesis / physiology*
|
| IF |
2.985
|
| 引用数 |
223
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
