RRC ID 43471
著者 Okamoto K, Muraguchi T, Shidoji Y.
タイトル Enhanced Glucose Requirement in Human Hepatoma-derived HuH-7 Cells by Forced Expression of the bcl-2 Gene.
ジャーナル J Clin Biochem Nutr
Abstract To explore the metabolic effects of Bcl-2 in tumor cells, a stable clone of HuH-7/bcl-2 and its control HuH-7/neo were established. Mitochondrial localization of ectopic Bcl-2 was demonstrated both by western blotting and immunofluorescence. HuH-7/bcl-2 cells consumed glucose at a higher rate, exhausted the available cellular ATP and died on day 9, while HuH-7/neo cells were still alive for 10 days under the same condition where cells were cultured without replenishment of the medium. The expression of the hexokinase II gene was up-regulated in HuH-7/bcl-2 at its protein level. Taken together, we suggest that the forced expression of Bcl-2 in human hepatoma may cause the cells to become more glucose-dependent for survival.
巻・号 43(2)
ページ 101-8
公開日 2008-9-1
DOI 10.3164/jcbn.2008053
PMID 18818743
PMC PMC2533714
IF 2.405
引用数 2
WOS 分野 NUTRITION & DIETETICS
リソース情報
ヒト・動物細胞 HuH-7(RCB1366)