RRC ID 43747
著者 Hashimoto O, Takagi R, Yanuma F, Doi S, Shindo J, Endo H, Hasegawa Y, Shimasaki S.
タイトル Identification and characterization of canine growth differentiation factor-9 and its splicing variant.
ジャーナル Gene
Abstract Growth differentiation factor-9 (GDF-9), a member of the transforming growth factor-β (TGF-β) superfamily, is expressed exclusively in the oocyte within the ovary and plays essential roles in the ovarian function in mammals. However, a possible involvement of GDF-9 in canine ovarian physiology that has a unique ovulation process among mammals has not been studied. Interestingly, we have isolated two types of cDNA clones generated by an alternative splicing from a canine ovarian total RNA. The predominant long form cDNA shares a common precursor structure with GDF-9s in other species whereas the minor short form cDNA has a 172 amino acid truncation in the proregion. Using a transient expression system, we found that the long form cDNA has a defect in mature protein production whereas the short form cDNA readily produces mature protein. However, mutations at one or two N-glycosylation sites in the mature domain of the short form GDF-9 caused a loss in mature protein production. These results suggest that the prodomain and N-linked glycosylation of the mature domain regulate proper processing and secretion of canine GDF-9. Based on the biological functions of GDF-9, these characteristics of canine GDF-9 could be causatively linked to the unique ovulation process in the Canidae.
巻・号 499(2)
ページ 266-72
公開日 2012-3-27
DOI 10.1016/j.gene.2012.03.003
PII S0378-1119(12)00301-0
PMID 22446043
PMC PMC3367864
MeSH Amino Acid Sequence Animals Cloning, Molecular Dogs / metabolism* Female Glycosylation Growth Differentiation Factor 9 / chemistry* Growth Differentiation Factor 9 / genetics Growth Differentiation Factor 9 / isolation & purification* Growth Differentiation Factor 9 / metabolism Molecular Sequence Data Ovary / metabolism Protein Processing, Post-Translational RNA Splicing Recombinant Proteins / genetics Sequence Alignment
IF 2.415
引用数 3