RRC ID 44165
著者 Shinozuka E, Miyashita M, Mizuguchi Y, Akagi I, Kikuchi K, Makino H, Matsutani T, Hagiwara N, Nomura T, Uchida E, Takizawa T.
タイトル SnoN/SKIL modulates proliferation through control of hsa-miR-720 transcription in esophageal cancer cells.
ジャーナル Biochem Biophys Res Commun
Abstract It is now evident that changes in microRNA are involved in cancer progression, but the mechanisms of transcriptional regulation of miRNAs remain unknown. Ski-related novel gene (SnoN/SKIL), a transcription co-factor, acts as a potential key regulator within a complex network of p53 transcriptional repressors. SnoN has pro- and anti-oncogenic functions in the regulation of cell proliferation, senescence, apoptosis, and differentiation. We characterized the roles of SnoN in miRNA transcriptional regulation and its effects on cell proliferation using esophageal squamous cell carcinoma (ESCC) cells. Silencing of SnoN altered a set of miRNA expression profiles in TE-1cells, and the expression levels of miR-720, miR-1274A, and miR-1274B were modulated by SnoN. The expression of these miRNAs resulted in changes to the target protein p63 and a disintegrin and metalloproteinase domain 9 (ADAM9). Furthermore, silencing of SnoN significantly upregulated cell proliferation in TE-1 cells, indicating a potential anti-oncogenic function. These results support our observation that cancer tissues have lower expression levels of SnoN, miR-720, and miR-1274A compared to adjacent normal tissues from ESCC patients. These data demonstrate a novel mechanism of miRNA regulation, leading to changes in cell proliferation.
巻・号 430(1)
ページ 101-6
公開日 2013-1-4
DOI 10.1016/j.bbrc.2012.11.005
PII S0006-291X(12)02146-8
PMID 23154181
MeSH Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / pathology* Cell Line, Tumor Cell Proliferation Esophageal Neoplasms / genetics Esophageal Neoplasms / pathology* Gene Expression Regulation, Neoplastic* Humans Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* MicroRNAs / genetics* Oligonucleotide Array Sequence Analysis Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism* RNA, Small Interfering / genetics Transcription, Genetic*
IF 2.985
引用数 16
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞