RRC ID |
44165
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著者 |
Shinozuka E, Miyashita M, Mizuguchi Y, Akagi I, Kikuchi K, Makino H, Matsutani T, Hagiwara N, Nomura T, Uchida E, Takizawa T.
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タイトル |
SnoN/SKIL modulates proliferation through control of hsa-miR-720 transcription in esophageal cancer cells.
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ジャーナル |
Biochem Biophys Res Commun
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Abstract |
It is now evident that changes in microRNA are involved in cancer progression, but the mechanisms of transcriptional regulation of miRNAs remain unknown. Ski-related novel gene (SnoN/SKIL), a transcription co-factor, acts as a potential key regulator within a complex network of p53 transcriptional repressors. SnoN has pro- and anti-oncogenic functions in the regulation of cell proliferation, senescence, apoptosis, and differentiation. We characterized the roles of SnoN in miRNA transcriptional regulation and its effects on cell proliferation using esophageal squamous cell carcinoma (ESCC) cells. Silencing of SnoN altered a set of miRNA expression profiles in TE-1cells, and the expression levels of miR-720, miR-1274A, and miR-1274B were modulated by SnoN. The expression of these miRNAs resulted in changes to the target protein p63 and a disintegrin and metalloproteinase domain 9 (ADAM9). Furthermore, silencing of SnoN significantly upregulated cell proliferation in TE-1 cells, indicating a potential anti-oncogenic function. These results support our observation that cancer tissues have lower expression levels of SnoN, miR-720, and miR-1274A compared to adjacent normal tissues from ESCC patients. These data demonstrate a novel mechanism of miRNA regulation, leading to changes in cell proliferation.
|
巻・号 |
430(1)
|
ページ |
101-6
|
公開日 |
2013-1-4
|
DOI |
10.1016/j.bbrc.2012.11.005
|
PII |
S0006-291X(12)02146-8
|
PMID |
23154181
|
MeSH |
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Cell Proliferation
Esophageal Neoplasms / genetics
Esophageal Neoplasms / pathology*
Gene Expression Regulation, Neoplastic*
Humans
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism*
MicroRNAs / genetics*
Oligonucleotide Array Sequence Analysis
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism*
RNA, Small Interfering / genetics
Transcription, Genetic*
|
IF |
2.985
|
引用数 |
16
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|