RRC ID |
443
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著者 |
Quan GX, Kim I, Kômoto N, Sezutsu H, Ote M, Shimada T, Kanda T, Mita K, Kobayashi M, Tamura T.
|
タイトル |
Characterization of the kynurenine 3-monooxygenase gene corresponding to the white egg 1 mutant in the silkworm Bombyx mori.
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ジャーナル |
Mol Genet Genomics
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Abstract |
Kynurenine 3-monooxygenase (KMO, EC 1.14.13.9), which catalyzes the oxidation of kynurenine to 3-hydroxykynurenine, is involved in the synthesis of ommochrome pigments in insects. A silkworm mutant, white egg 1 ( w-1), has been shown to be deficient in this enzyme activity. The mutant is characterized morphologically by its white eyes and the fact that the females lay white eggs. To analyze the relationship between the KMO gene and the mutation, we first determined the entire sequence of a full-length 2.0-kb cDNA and examined its expression pattern in the wild type. The cDNA sequence contains one ORF encoding a polypeptide of 456 amino acids, and transcripts were detected in the larval Malpighian tubules and the pupal ovaries, but not in other tissues. Southern analysis and nucleotide sequencing showed that the KMO gene is present in a single copy and consists of ten exons distributed over a 16-kb region. Comparison of the transcripts between the wild type and mutant silkworms showed that the wild type expressed a single transcript, whereas the mutant exhibited markedly reduced amounts of two transcripts with sizes of 2.0 kb and 1.8 kb. Nucleotide sequence analysis of these mutant transcripts indicated that sequences corresponding to the ninth and tenth exons were missing. Inverse PCR and Southern analysis of the mutant gene demonstrated that the corresponding genomic region was deleted in the w-1 mutant.
|
巻・号 |
267(1)
|
ページ |
1-9
|
公開日 |
2002-3-1
|
DOI |
10.1007/s00438-001-0629-2
|
解説 |
w-1(第1白卵)の原因遺伝子はBmKMOである。BmKMOはキヌレニン酸化酵素をコードする。w-1系統では、BmKMOのエクソン9と10が欠失している。
|
PMID |
11919709
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Bombyx / genetics*
Chromosome Mapping
DNA, Complementary
Gene Expression
Kynurenine 3-Monooxygenase
Mixed Function Oxygenases / genetics*
Molecular Sequence Data
Mutation*
Open Reading Frames
Sequence Homology, Amino Acid
|
IF |
2.797
|
引用数 |
30
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
カイコ |
w-1 mutant
w-2 mutant
fl mutant ? |