RRC ID 45120
著者 Nagano K, Yamashita T, Inoue M, Higashisaka K, Yoshioka Y, Abe Y, Mukai Y, Kamada H, Tsutsumi Y, Tsunoda S.
タイトル Eph receptor A10 has a potential as a target for a prostate cancer therapy.
ジャーナル Biochem Biophys Res Commun
Abstract We recently identified Eph receptor A10 (EphA10) as a novel breast cancer-specific protein. Moreover, we also showed that an in-house developed anti-EphA10 monoclonal antibody (mAb) significantly inhibited proliferation of breast cancer cells, suggesting EphA10 as a promising target for breast cancer therapy. However, the only other known report for EphA10 was its expression in the testis at the mRNA level. Therefore, the potency of EphA10 as a drug target against cancers other than the breast is not known. The expression of EphA10 in a wide variety of cancer cells was studied and the potential of EphA10 as a drug target was evaluated. Screening of EphA10 mRNA expression showed that EphA10 was overexpressed in breast cancer cell lines as well as in prostate and colon cancer cell lines. Thus, we focused on prostate cancers in which EphA10 expression was equivalent to that in breast cancers. As a result, EphA10 expression was clearly shown in clinical prostate tumor tissues as well as in cell lines at the mRNA and protein levels. In order to evaluate the potential of EphA10 as a drug target, we analyzed complement-dependent cytotoxicity effects of anti-EphA10 mAb and found that significant cytotoxicity was mediated by the expression of EphA10. Therefore, the idea was conceived that the overexpression of EphA10 in prostate cancers might have a potential as a target for prostate cancer therapy, and formed the basis for the studies reported here.
巻・号 450(1)
ページ 545-9
公開日 2014-7-18
DOI 10.1016/j.bbrc.2014.06.007
PII S0006-291X(14)01059-6
PMID 24924629
MeSH Antibodies, Monoclonal / immunology Antibodies, Monoclonal / therapeutic use* Antineoplastic Agents / immunology Antineoplastic Agents / therapeutic use Biomarkers, Tumor / metabolism* Breast Neoplasms / drug therapy Breast Neoplasms / metabolism* Breast Neoplasms / pathology Cell Line, Tumor Cell Survival Colonic Neoplasms / drug therapy Colonic Neoplasms / metabolism* Colonic Neoplasms / pathology Drug Delivery Systems / methods Female Humans Male Prostatic Neoplasms / drug therapy* Prostatic Neoplasms / metabolism* Prostatic Neoplasms / pathology Receptors, Eph Family / immunology Receptors, Eph Family / metabolism* Treatment Outcome
IF 2.985
引用数 13
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞