RRC ID 45674
著者 Alriyami MZ, Jones MR, Johnsen RC, Banerjee Y, Baillie DL.
タイトル let-65 is cytoplasmic methionyl tRNA synthetase in C. elegans.
ジャーナル Meta Gene
Abstract Cytoplasmic methionyl tRNA synthetase (MetRS) is one of more than 20 cytoplasmic aminoacyl tRNA synthetase enzymes (ARS). This family of enzymes catalyzes a process fundamental for protein translation. Using a combination of genetic mapping, oligonucleotide array comparative genomic hybridization, and phenotypic correlation, we show that mutations in the essential gene, let-65, reside within the predicted Caenorhabditis elegans homologue of MetRS, which we have named mars-1. We demonstrate that the lethality associated with alleles of let-65 is fully rescued by a transgenic array that spans the mars-1 genomic region. Furthermore, sequence analysis reveals that six let-65 alleles lead to the alteration of highly conserved amino acids.
巻・号 2
ページ 819-30
公開日 2014-12-1
DOI 10.1016/j.mgene.2014.08.006
PII S2214-5400(14)00069-3
PMID 25606464
PMC PMC4287814
引用数 0
リソース情報
線虫 tm3799 tm4074 tm5774 tm524 tm3947