RRC ID 46078
著者 Manil-Ségalen M, Lefebvre C, Jenzer C, Trichet M, Boulogne C, Satiat-Jeunemaitre B, Legouis R.
タイトル The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by interacting with the HOPS subunit VPS39.
ジャーナル Dev Cell
Abstract The formation of the autophagic vesicles requires the recruitment of ubiquitin-like Atg8 proteins to the membrane of nascent autophagosomes. Seven Atg8 homologs are present in mammals, split into the LC3 and the GABARAP/GATE-16 families, whose respective functions are unknown. Using Caenorhabditis elegans, we investigated the functions of the GABARAP and the LC3 homologs, LGG-1 and LGG-2, in autophagosome biogenesis. Both LGG-1 and LGG-2 localize to the autophagosomes but display partially overlapping patterns. During allophagy, a developmentally stereotyped autophagic flux, LGG-1 acts upstream of LGG-2 to allow its localization to autophagosomes. LGG-2 controls the maturation of LGG-1-positive autophagosomes and facilitates the tethering with the lysosomes through a direct interaction with the VPS-39 HOPS complex subunit. Genetic analyses sustain a sequential implication of LGG-1, LGG-2, RAB-7, and HOPS complex to generate autolysosomes. The duplications of Atg8 in metazoans thus allowed the acquisition of specialized functions for autophagosome maturation.
巻・号 28(1)
ページ 43-55
公開日 2014-1-13
DOI 10.1016/j.devcel.2013.11.022
PII S1534-5807(13)00703-X
PMID 24374177
MeSH Animals Autophagy* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Lysosomes / metabolism Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Phagosomes / metabolism* Protein Binding Protein Subunits / genetics Protein Subunits / metabolism Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism*
IF 10.092
引用数 63
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm3489 tm5755 tm2253