RRC ID 49379
著者 Wang MQ, Sun L.
タイトル Pseudomonas oceani sp. nov., isolated from deep seawater.
ジャーナル Int J Syst Evol Microbiol
Abstract In this study, we identified a novel Gram-stain-negative, aerobic, motile, and rod-shaped bacterium, strain KX 20T, isolated from the deep seawater in Okinawa Trough, northwestern Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain KX 20T was related to members of the genus Pseudomonas and shares the highest sequence identities with Pseudomonas aestusnigri CECT 8317T (99.4 %) and Pseudomonas pachastrellae JCM 12285T (98.5 %). The 16S rRNA gene sequence identities between strain KX 20T and other members of the genus Pseudomonaswere below 96.6 %. The gyrB and rpoD genes of strain KX 20T shared 82.0 to 89.3 % sequence identity with the gyrB and rpoD genes of the closest phylogenetic neighbours of KX 20T. The predominant cellular fatty acids of strain KX 20T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (29.2 %), C16 : 0 (24.5 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (21.5 %) and C12 : 0 (8.2 %). The major polar lipids of strain KX 20T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unknown phospholipids. The genomic DNA G+C content of strain KX 20T was 62.9 mol%. On the basis of phylogenetic analysis and phenotypic characteristics, a novel species, Pseudomonas oceani sp. nov. is proposed. The type strain is KX 20T (=CGMCC 1.15195T=DSM 100277T).
巻・号 66(10)
ページ 4250-4255
公開日 2016-10-1
DOI 10.1099/ijsem.0.001343
PMID 27468709
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Genes, Bacterial Nucleic Acid Hybridization Pacific Ocean Phospholipids / chemistry Phylogeny* Pseudomonas / classification* Pseudomonas / genetics Pseudomonas / isolation & purification RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA
IF 2.415
引用数 8
リソース情報
一般微生物 JCM 12285