RRC ID 5067
Author Lee SD.
Title Marmoricola aequoreus sp. nov., a novel actinobacterium isolated from marine sediment.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A yellow-coloured, marine actinobacterium, designated SST-45(T), was isolated from sandy sediment under the surface of a beach and taxonomically characterized by physiological, chemotaxonomic and phylogenetic methods. The cells of the isolate were Gram-positive, aerobic, non-sporulating, non-motile, spherical cells that occurred singly, in pairs, in clusters or as short chains. The isolate grew at 10-37 degrees C, an initial pH 5.1-12.1 and in the presence of 5 % (w/v) NaCl. The organism possessed LL-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell wall, MK-8(H(4)) as the major menaquinone, a polar lipid profile including diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid, C(18 : 1) and C(16 : 0) as the major fatty acids, and a DNA G+C content of 72.4 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that the organism was related to members of the genera Marmoricola and Nocardioides. The closest neighbours were Marmoricola aurantiacus DSM 12652(T) (97.0 % sequence similarity) and Nocardioides jensenii KCTC 9134(T) (96.7 %). The combination of morphological and chemotaxonomic characters supported the assignment of the isolate to the genus Marmoricola. However, the organism is clearly distinguished phenotypically from the single described species of this genus, Marmoricola aurantiacus. Based on the data obtained, the organism has been assigned as a novel species, for which the name Marmoricola aequoreus sp. nov. is proposed. The type strain is strain SST-45(T) (=JCM 13812(T)=NRRL B-24464(T)).
Volume 57(Pt 7)
Pages 1391-5
Published 2007-7
DOI 10.1099/ijs.0.64696-0
PII 57/7/1391
PMID 17625163
MeSH Actinomycetales / chemistry Actinomycetales / classification* Actinomycetales / isolation & purification* Actinomycetales / physiology Aerobiosis Base Composition Cell Wall / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Fatty Acids / analysis Genes, rRNA Geologic Sediments / microbiology* Hydrogen-Ion Concentration Molecular Sequence Data Phospholipids / analysis Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Temperature Vitamin K 2 / analysis
IF 1.932
Times Cited 14
WOS Category MICROBIOLOGY
Resource
General Microbes JCM13812