RRC ID 51151
著者 Okiria J, Ten LN, Lee JJ, Lee SY, Cho YJ, Kim MK, Jung HY.
タイトル Spirosoma litoris sp. nov., a bacterium isolated from beach soil.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J16-2T3AT, was isolated from beach soil on Jeju Island, South Korea. Strain 15J16-2T3AT grew at 10-37 °C (optimum growth at 25 °C) and pH 6.5-8.5 (optimum growth at pH 7). Based on 16S rRNA gene phylogenetic analysis, the novel strain was closely related to members of the genus Spirosoma(94.8-89.9 % similarities) and formed a separate branch within the genus together with Spirosoma luteolum 16F6ET in neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees. The G+C content of the genomic DNA of strain 15J16-2T3AT was 47.6 mol%. The detection of menaquinone MK-7 as the predominant respiratory quinone, a fatty acid profile with summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 35.5 %), C16 : 1ω5c (26.6 %), and iso C15 : 0 (10.1 %) as the major components, phosphatidylethanolamine and unidentified aminophospholipid as the major polar lipids also support the affiliation of strain 15J16-2T3AT with the genus Spirosoma. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J16-2T3AT from members the genus Spirosoma. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain 15J16-2T3AT represents a novel species of the genus Spirosoma, for which the name Spirosoma litoris sp. nov. is proposed. The type strain is 15J16-2T3AT (=KCTC 52029T=JCM 31999T).
巻・号 67(12)
ページ 4986-4991
公開日 2017-12-1
DOI 10.1099/ijsem.0.002394
PMID 29058659
MeSH Bacterial Typing Techniques Base Composition Cytophagaceae / classification* Cytophagaceae / genetics Cytophagaceae / isolation & purification DNA, Bacterial / genetics Fatty Acids / chemistry Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Republic of Korea Sequence Analysis, DNA Soil Microbiology* Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 0
リソース情報
一般微生物 JCM 31999