| RRC ID |
51438
|
| 著者 |
Liu W, Yu E, Chen S, Ma X, Lu Y, Liu X.
|
| タイトル |
Spatiotemporal expression profiling of long intervening noncoding RNAs in Caenorhabditis elegans.
|
| ジャーナル |
Sci Rep
|
| Abstract |
To better understand the biological function of long noncoding RNAs, it is critical to determine their spatiotemporal expression patterns. We generated transgenic reporter strains for 149 out of the 170 annotated C. elegans long intervening noncoding RNAs (lincRNAs) and profiled their temporal activity. For the 68 lincRNAs with integrated reporter lines, we profiled their expression at the resolution of single cells in L1 larvae, and revealed that the expression of lincRNAs is more specific, heterogeneous and at lower level than transcription factors (TFs). These expression patterns can be largely attributed to transcriptional regulation because they were observed in assays using reporters of promoter activity. The spatial expression patterns of the 68 lincRNAs were further examined in 18 tissue categories throughout eight developmental stages. We compared the expression dynamics of lincRNAs, miRNAs and TFs during development. lincRNA and miRNA promoters are less active at embryo stage than those of TFs, but become comparable to TFs after embryogenesis. Finally, the lincRNA gene set shows a similar tissue distribution to that of miRNAs and TFs. We also generated a database, CELE, for the storage and retrieval of lincRNA reporter expression patterns and other relevant information. The data and strains described here will provide a valuable guide and resource for future functional exploration of C. elegans lincRNAs.
|
| 巻・号 |
7(1)
|
| ページ |
5195
|
| 公開日 |
2017-7-12
|
| DOI |
10.1038/s41598-017-05427-5
|
| PII |
10.1038/s41598-017-05427-5
|
| PMID |
28701691
|
| PMC |
PMC5507858
|
| MeSH |
Animals
Caenorhabditis elegans / genetics*
Fluorescent Antibody Technique
Gene Expression
Gene Expression Profiling*
Genes, Reporter
Promoter Regions, Genetic
RNA, Long Noncoding / genetics*
Single-Cell Analysis
Transcription Factors / genetics
Transcriptome*
|
| IF |
3.998
|
| 引用数 |
4
|
| リソース情報 |
| 線虫 |
tm4063 |
