RRC ID 51595
著者 Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.
タイトル Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.
ジャーナル Nat Commun
Abstract Anterior pituitary is critical for endocrine systems. Its hormonal responses to positive and negative regulators are indispensable for homeostasis. For this reason, generating human anterior pituitary tissue that retains regulatory hormonal control in vitro is an important step for the development of cell transplantation therapy for pituitary diseases. Here we achieve this by recapitulating mouse pituitary development using human embryonic stem cells. We find that anterior pituitary self-forms in vitro following the co-induction of hypothalamic and oral ectoderm. The juxtaposition of these tissues facilitated the formation of pituitary placode, which subsequently differentiated into pituitary hormone-producing cells. They responded normally to both releasing and feedback signals. In addition, after transplantation into hypopituitary mice, the in vitro-generated corticotrophs rescued physical activity levels and survival of the hosts. Thus, we report a useful methodology for the production of regulator-responsive human pituitary tissue that may benefit future studies in regenerative medicine.
巻・号 7
ページ 10351
公開日 2016-1-14
DOI 10.1038/ncomms10351
PII ncomms10351
PMID 26762480
PMC PMC4735598
MeSH Adrenocorticotropic Hormone / metabolism Animals Cell Culture Techniques Corticotrophs / transplantation* Embryonic Development Embryonic Stem Cells / physiology* Growth Hormone / metabolism Humans Hypopituitarism / therapy* Mice Pituitary Gland, Anterior / embryology* Pituitary Gland, Anterior / metabolism Tissue Engineering / methods*
IF 12.121
引用数 55
リソース情報
ヒト・動物細胞 KhES-1(HES0001) KhES-3(HES0003)