RRC ID |
51595
|
著者 |
Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.
|
タイトル |
Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.
|
ジャーナル |
Nat Commun
|
Abstract |
Anterior pituitary is critical for endocrine systems. Its hormonal responses to positive and negative regulators are indispensable for homeostasis. For this reason, generating human anterior pituitary tissue that retains regulatory hormonal control in vitro is an important step for the development of cell transplantation therapy for pituitary diseases. Here we achieve this by recapitulating mouse pituitary development using human embryonic stem cells. We find that anterior pituitary self-forms in vitro following the co-induction of hypothalamic and oral ectoderm. The juxtaposition of these tissues facilitated the formation of pituitary placode, which subsequently differentiated into pituitary hormone-producing cells. They responded normally to both releasing and feedback signals. In addition, after transplantation into hypopituitary mice, the in vitro-generated corticotrophs rescued physical activity levels and survival of the hosts. Thus, we report a useful methodology for the production of regulator-responsive human pituitary tissue that may benefit future studies in regenerative medicine.
|
巻・号 |
7
|
ページ |
10351
|
公開日 |
2016-1-14
|
DOI |
10.1038/ncomms10351
|
PII |
ncomms10351
|
PMID |
26762480
|
PMC |
PMC4735598
|
MeSH |
Adrenocorticotropic Hormone / metabolism
Animals
Cell Culture Techniques
Corticotrophs / transplantation*
Embryonic Development
Embryonic Stem Cells / physiology*
Growth Hormone / metabolism
Humans
Hypopituitarism / therapy*
Mice
Pituitary Gland, Anterior / embryology*
Pituitary Gland, Anterior / metabolism
Tissue Engineering / methods*
|
IF |
12.121
|
引用数 |
55
|
リソース情報 |
ヒト・動物細胞 |
KhES-1(HES0001)
KhES-3(HES0003) |