Reference - Detail
| RRC ID | 52445 |
|---|---|
| Author | Sakaue-Sawano A, Yo M, Komatsu N, Hiratsuka T, Kogure T, Hoshida T, Goshima N, Matsuda M, Miyoshi H, Miyawaki A. |
| Title | Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. |
| Journal | Mol. Cell |
| Abstract |
-mediated ubiquitylation. In cultured cells, Fucci(CA) produced a sharp triple color-distinct separation of G1, S, and G2, while Fucci(SCA) permitted a two-color readout of G1 and S/G2. Fucci(CA) applications included tracking the transient G1 phase of rapidly dividing mouse embryonic stem cells and identifying a window for UV-irradiation damage in S phase. These results show that Fucci(CA) is an essential tool for quantitative studies of interphase cell-cycle regulation. |
| Volume | 68(3) |
| Pages | 626-640.e5 |
| Published | 2017-11-2 |
| DOI | 10.1016/j.molcel.2017.10.001 |
| PII | S1097-2765(17)30750-5 |
| PMID | 29107535 |
| MeSH | Animals Cell Cycle / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cells, Cultured Cullin Proteins / genetics Cullin Proteins / metabolism* Embryonic Stem Cells / cytology Embryonic Stem Cells / physiology* Fluorescent Dyes / metabolism* Genes, Reporter HeLa Cells Humans Luminescent Proteins / genetics Luminescent Proteins / metabolism* Mice |
| IF | 14.248 |
| Resource | |
| Human and Animal Cells | HeLa/Fucci2(RCB2867) |