RRC ID 53242
Author Ren Z, Veksler-Lublinsky I, Morrissey D, Ambros V.
Title Staufen Negatively Modulates MicroRNA Activity in Caenorhabditis elegans.
Journal G3 (Bethesda)
Abstract The double-stranded RNA-binding protein Staufen has been implicated in various posttranscriptional gene regulatory processes. Here, we demonstrate that the Caenorhabditis elegans homolog of Staufen, STAU-1, functionally interacts with microRNAs. Loss-of-function mutations of stau-1 significantly suppress phenotypes of let-7 family microRNA mutants, a hypomorphic allele of dicer, and a lsy-6 microRNA partial loss-of-function mutant. Furthermore, STAU-1 modulates the activity of lin-14, a target of lin-4 and let-7 family microRNAs, and this modulation is abolished when the 3' untranslated region of lin-14 is removed. Deep sequencing of small RNA cDNA libraries reveals no dramatic change in the levels of microRNAs or other small RNA populations between wild-type and stau-1 mutants, with the exception of certain endogenous siRNAs in the WAGO pathway. The modulation of microRNA activity by STAU-1 does not seem to be associated with the previously reported enhanced exogenous RNAi (Eri) phenotype of stau-1 mutants, since eri-1 exhibits the opposite effect on microRNA activity. Altogether, our results suggest that STAU-1 negatively modulates microRNA activity downstream of microRNA biogenesis, possibly by competing with microRNAs for binding on the 3' untranslated region of target mRNAs.
Volume 6(5)
Pages 1227-37
Published 2016-5-3
DOI 10.1534/g3.116.027300
PII g3.116.027300
PMID 26921297
PMC PMC4856075
MeSH 3' Untranslated Regions Alleles Animals CRISPR-Cas Systems Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Computational Biology / methods Gene Editing Gene Expression Regulation* Genetic Association Studies Genotype MicroRNAs / genetics* Mutation Phenotype RNA Interference RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism*
IF 2.781
Times Cited 5
C.elegans tm2266