RRC ID 53411
Author Palikaras K, Tavernarakis N.
Title Intracellular Assessment of ATP Levels in Caenorhabditis elegans.
Journal Bio Protoc
Abstract Eukaryotic cells heavily depend on adenosine triphosphate (ATP) generated by oxidative phosphorylation (OXPHOS) within mitochondria. ATP is the major energy currency molecule, which fuels cell to carry out numerous processes, including growth, differentiation, transportation and cell death among others (Khakh and Burnstock, 2009). Therefore, ATP levels can serve as a metabolic gauge for cellular homeostasis and survival (Artal-Sanz and Tavernarakis, 2009; Gomes et al., 2011; Palikaras et al., 2015). In this protocol, we describe a method for the determination of intracellular ATP levels using a bioluminescence approach in the nematode Caenorhabditis elegans.
Volume 6(23)
Published 2016-12-5
DOI 10.21769/BioProtoc.2048
PII e22048
PMID 28194429
PMC PMC5303341
Resource
C.elegans tm1779