| RRC ID |
54359
|
| Author |
Nakano A, Masuda K, Hiromoto T, Takahashi K, Matsumoto Y, Habib AG, Darwish AG, Yukawa M, Tsuchiya E, Ueno M.
|
| Title |
Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.
|
| Journal |
Mol Cell Biol
|
| Abstract |
The spindle assembly checkpoint (SAC) monitors defects in kinetochore-microtubule attachment or lack of tension at kinetochores and arrests cells at prometaphase. In fission yeast, the double mutant between pot1Δ and the helicase-dead point mutant of the RecQ helicase Rqh1 gene (rqh1-hd) accumulates Rad51-dependent recombination intermediates at telomeres and enters mitosis with those intermediates. Here, we found that SAC-dependent prometaphase arrest occurred more frequently in pot1Δ rqh1-hd double mutants than in rqh1-hd single mutants. SAC-dependent prometaphase arrest also occurred more frequently in rqh1-hd single mutants after cells were released from DNA replication block compared to the rqh1-hd single mutant in the absence of exogenous insult to the DNA. In both cases, Mad2 foci persisted longer than usual at kinetochores, suggesting a defect in kinetochore-microtubule attachment. In pot1Δ rqh1-hd double mutants and rqh1-hd single mutants released from DNA replication block, SAC-dependent prometaphase arrest was suppressed by the removal of the recombination or replication intermediates. Our results indicate that the accumulation of recombination or replication intermediates induces SAC-dependent prometaphase arrest, possibly by affecting kinetochore-microtubule attachment.
|
| Volume |
34(8)
|
| Pages |
1389-97
|
| Published |
2014-4-1
|
| DOI |
10.1128/MCB.01704-13
|
| PII |
MCB.01704-13
|
| PMID |
24469396
|
| PMC |
PMC3993586
|
| MeSH |
Animals
Cell Cycle Proteins / genetics
Chromosome Segregation / genetics
Chromosomes, Fungal / genetics
DNA Repair / genetics
DNA, Ribosomal / genetics*
Genes, cdc / genetics*
Kinetochores / metabolism
M Phase Cell Cycle Checkpoints / genetics
Mitosis / genetics
Mitosis / physiology
Mutation / genetics
Rad51 Recombinase / genetics*
Schizosaccharomyces / genetics*
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
Spindle Apparatus / genetics*
Telomere / genetics*
|
| IF |
3.611
|
| Times Cited |
4
|
| Resource |
| Yeast |
FY10134, FY18581, FY18537 |
