| RRC ID |
56386
|
| 著者 |
Yuji Ishida, Yukoh Hiei, Toshihiko Komari
|
| タイトル |
High Efficiency Wheat Transformation Mediated by Agrobacterium tumefaciens
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| ジャーナル |
Advances in Wheat Genetics: From Genome to Field, Y. Ogihara et al. (eds.), Springer Japan
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| Abstract |
Wheat is one of the most important cereals for humans but quite recalcitrant in transformation. We have thoroughly examined every aspect of the wheat trans-formation protocols mediated by Agrobacterium tumefaciens and we were able to identify and optimize the key factors. Immature embryos isolated from healthy plants grown in a greenhouse were pre-treated with centrifuging and co-cultivated with A. tumefaciens. The frequency of transformation (independent transgenics / explant) was between 50% and 60% were routinely observed and higher than 90% were recorded in the best cases. Not surprisingly, the key factors did not dif-fer much from those in other cereal plants such as rice and maize. Both bar and hpt genes were good as selection markers. Fielder, a spring wheat cultivar, con-stantly showed high efficiency of transformation by our protocol. We have been able to obtain transgenic plants from the embryos harvested from the greenhous-es throughout the year. Most of the transformed plants were normal in morphol-ogy and fully fertile. More than 40% of the transformants had a single copy of the transgenes, which were inherited in a Mendelian fashion in most of the lines analyzed. Transgenic wheat has been generated at high frequency by several re-search groups by our protocol by now. Therefore, wheat has finally joined the list of cereals that can be efficiently transformed.
|
| ページ |
pp 167-173
|
| 公開日 |
2015-1-1
|
| DOI |
DOI 10.1007/978-4-431-55675-6_18
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| リソース情報 |
| コムギ |
KT020-061 |
