| RRC ID |
56956
|
| 著者 |
Kidani S, Okuzaki Y, Kaneoka H, Asai S, Murakami S, Murase Y, Iijima S, Nishijima KI.
|
| タイトル |
Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections.
|
| ジャーナル |
Cytotechnology
|
| Abstract |
In mammals, interferon-inducible transmembrane proteins (IFITMs) prevent infections by various enveloped viruses. The expression of IFITMs in chicken was herein examined in the adult and embryonic organs using a quantitative reverse-transcription-polymerase chain reaction. The results obtained revealed that IFITM3 was expressed at a higher level than IFITM1, 2 and 5, in both embryonic and adult organs. However, the expression levels of IFITMs in embryonic organs were less than 5 % of those in adult lungs. Among the embryonic tissues examined, primordial germ cells (PGCs) at day 2.5 expressed relatively higher levels of IFITM3. IFITM3 expression levels were 1.5-fold higher in the chicken cell line DF-1 than in PGCs. The knockdown of IFITM3 in DF-1 cells by siRNA increased the infectivity of a vesicular stomatitis virus G protein-pseudotyped lentiviral vector, suggesting that lower levels of IFITM3 are still sufficient to restrict this viral vector.
|
| 巻・号 |
69(3)
|
| ページ |
477-484
|
| 公開日 |
2017-6-1
|
| DOI |
10.1007/s10616-016-9958-1
|
| PII |
10.1007/s10616-016-9958-1
|
| PMID |
27117301
|
| PMC |
PMC5461238
|
| IF |
1.672
|
| 引用数 |
2
|
| リソース情報 |
| ニワトリ・ウズラ |
|
