論文 - 詳細
| RRC ID | 57835 |
|---|---|
| 著者 | Miura N, Ishihara Y, Miura Y, Kimoto M, Miura K. |
| タイトル | miR-520d-5p can reduce the mutations in hepatoma cancer cells and iPSCs-derivatives. |
| ジャーナル | BMC Cancer |
| Abstract |
BACKGROUND:Human microRNAs (miRNAs) have diverse functions in biology, and play a role in nearly every biological process. Here we report that miR-520d-5p (520d-5p) causes undifferentiated cancer cells to adopt benign or normal status in vivo in immunodeficient mice via demethylation and P53 upregulation. Further we found that 520-5p causes normal cells to elongate cellular lifetime and mesenchymal stem cell-like status with CD105 positivity. We hypothesized that ectopic 520d-5p expression reduced mutations in undifferentiated type of hepatoma (HLF) cells through synergistic modulation of methylation-related enzymatic expression. METHODS:To examine whether there were any changes in mutation status in cells treated with 520d-5p, we performed next generation sequencing (NGS) in HLF cells and human iPSC-derivative cells in pre-mesenchymal stem cell status. We analyzed the data using both genome-wide and individual gene function approaches. RESULTS:520d-5p induced a shift towards a wild type or non-malignant phenotype, which was regulated by nucleotide mutations in both HLF cells and iPSCs. Further, 520d-5p reduced mutation levels in both the whole genome and genomic fragment assemblies. CONCLUSIONS:Cancer cell genomic mutations cannot be repaired in most contexts. However, these findings suggest that applied development of 520d-5p would allow new approaches to cancer research and improve the quality of iPSCs used in regenerative medicine. |
| 巻・号 | 19(1) |
| ページ | 587 |
| 公開日 | 2019-6-15 |
| DOI | 10.1186/s12885-019-5786-y |
| PII | 10.1186/s12885-019-5786-y |
| PMID | 31202279 |
| PMC | PMC6570841 |
| MeSH | Carcinoma, Hepatocellular / genetics* Carcinoma, Hepatocellular / pathology Cell Line, Transformed DNA-Binding Proteins / metabolism Gene Expression Regulation, Neoplastic Humans Induced Pluripotent Stem Cells* / metabolism Liver Neoplasms / genetics* Liver Neoplasms / pathology Mesenchymal Stem Cells / metabolism MicroRNAs / genetics* Mutation |
| IF | 3.15 |
| 引用数 | 2 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |