| RRC ID |
60034
|
| 著者 |
Ishida H, Haniu H, Takeuchi A, Ueda K, Sano M, Tanaka M, Takizawa T, Sobajima A, Kamanaka T, Saito N.
|
| タイトル |
In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material.
|
| ジャーナル |
Materials (Basel)
|
| Abstract |
We evaluated starfish-derived β-tricalcium phosphate (Sf-TCP) obtained by phosphatization of starfish-bone-derived porous calcium carbonate as a potential bone substitute material. The Sf-TCP had a communicating pore structure with a pore size of approximately 10 μm. Although the porosity of Sf-TCP was similar to that of Cerasorb M (CM)-a commercially available β-TCP bone filler-the specific surface area was roughly three times larger than that of CM. Observation by scanning electron microscopy showed that pores communicated to the inside of the Sf-TCP. Cell growth tests showed that Sf-TCP improved cell proliferation compared with CM. Cells grown on Sf-TCP showed stretched filopodia and adhered; cells migrated both to the surface and into pores. In vivo, vigorous tissue invasion into pores was observed in Sf-TCP, and more fibrous tissue was observed for Sf-TCP than CM. Moreover, capillary formation into pores was observed for Sf-TCP. Thus, Sf-TCP showed excellent biocompatibility in vitro and more vigorous bone formation in vivo, indicating the possible applications of this material as a bone substitute. In addition, our findings suggested that mimicking the microstructure derived from whole organisms may facilitate the development of superior artificial bone.
|
| 巻・号 |
12(11)
|
| 公開日 |
2019-6-11
|
| DOI |
10.3390/ma12111881
|
| PII |
ma12111881
|
| PMID |
31212709
|
| PMC |
PMC6601278
|
| IF |
3.057
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
