RRC ID 60062
著者 Wan Y, Wei Z, Looger LL, Koyama M, Druckmann S, Keller PJ.
タイトル Single-Cell Reconstruction of Emerging Population Activity in an Entire Developing Circuit.
ジャーナル Cell
Abstract Animal survival requires a functioning nervous system to develop during embryogenesis. Newborn neurons must assemble into circuits producing activity patterns capable of instructing behaviors. Elucidating how this process is coordinated requires new methods that follow maturation and activity of all cells across a developing circuit. We present an imaging method for comprehensively tracking neuron lineages, movements, molecular identities, and activity in the entire developing zebrafish spinal cord, from neurogenesis until the emergence of patterned activity instructing the earliest spontaneous motor behavior. We found that motoneurons are active first and form local patterned ensembles with neighboring neurons. These ensembles merge, synchronize globally after reaching a threshold size, and finally recruit commissural interneurons to orchestrate the left-right alternating patterns important for locomotion in vertebrates. Individual neurons undergo functional maturation stereotypically based on their birth time and anatomical origin. Our study provides a general strategy for reconstructing how functioning circuits emerge during embryogenesis. VIDEO ABSTRACT.
巻・号 179(2)
ページ 355-372.e23
公開日 2019-10-3
DOI 10.1016/j.cell.2019.08.039
PII S0092-8674(19)30958-4
PMID 31564455
PMC PMC7055533
IF 36.216
引用数 14
リソース情報
ゼブラフィッシュ Tg(vglut2a:lRl-ChR)