RRC ID 60530
Author Wang C, Lv Y, Li A, Feng G, Bao G, Zhu H, Tan Z.
Title Chitinophaga silvisoli sp. nov., isolated from forest soil.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A Gram-stain-negative, rod-shaped and aerobic bacterium, designated K20C18050901T, was isolated from forest soil collected on 11 September 2017 from Dinghushan Biosphere Reserve, Guangdong Province, PR China (23° 10' 24'' N; 112° 32' 10'' E). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain K20C18050901T belongs to the genus Chitinophaga, and showed the highest similarities to Chitinophaga sancti NBRC 15057T (98.6 %) and Chitinophaga oryziterrae JCM 16595T (96.9 %). The major fatty acids (>10 %) were iso-C15 : 0, C16 : 1ω5c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C17 : 0 3-OH. The predominant respiratory quinone was menaquinone-7. The major polar lipid was phosphatidylethanolamine. The draft genome size of strain K20C18050901T was 8.36 Mb with a DNA G+C content of 44.7 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain K20C18050901T and C. sancti NBRC 15057T were 31.40 and 85.82 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analysis, strain K20C18050901T represents a novel species of the genus Chitinophaga, for which the name Chitinophagasilvisoli sp. nov. is proposed. The type strain is K20C18050901T (=GDMCC 1.1411T=KCTC 62860T).
Volume 69(4)
Pages 909-913
Published 2019-4
DOI 10.1099/ijsem.0.003212
PMID 30608225
MeSH Bacterial Typing Techniques Bacteroidetes / classification* Bacteroidetes / isolation & purification Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Forests* Nucleic Acid Hybridization Phosphatidylethanolamines / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.166
Times Cited 1
General Microbes JCM16595