RRC ID 609
Author Iwase A, Ishii H, Aoyagi H, Ohme-Takagi M, Tanaka H.
Title Comparative analyses of the gene expression profiles of Arabidopsis intact plant and cultured cells.
Journal Biotechnol Lett
Abstract To characterize de-differentiated plant cultured cells and to determine factor(s) that regulate the de-differentiated state, comparative analyses of the gene expression profiles of intact plants and three different cultured cell lines of Arabidopsis were performed by DNA microarray and RNA blot analyses. The three different cultured cell lines (T87 and two cell lines newly induced from leaf and root tissue, respectively) were used. Twenty-seven to 140 genes were down-regulated but only 9-27 genes were up-regulated in each cultured cell lines when compared to the intact plants. Genes commonly regulated in each cultured cell lines were investigated and six genes, including those related to metabolism, were found to be down-regulated and two genes, that include those related to a transcription factor, were up-regulated.
Volume 27(15)
Pages 1097-103
Published 2005-8-1
DOI 10.1007/s10529-005-8456-x
PMID 16132859
MeSH Arabidopsis / genetics Arabidopsis Proteins / metabolism Cell Differentiation Cell Line Cells, Cultured DNA / chemistry DNA / metabolism Down-Regulation Gene Expression Profiling Gene Expression Regulation Gene Expression Regulation, Plant* Genes, Plant Oligonucleotide Array Sequence Analysis Plant Leaves / metabolism RNA / chemistry RNA / metabolism Transcription, Genetic Up-Regulation
IF 1.977
Times Cited 5
Arabidopsis / Cultured plant cells, genes rpc00008