RRC ID |
61095
|
著者 |
Kenta Watanabe, Yusei Higuchi, Mizuki Shimmura, Masato Tachibana, Masahiro Fujishima, Takashi Shimizu, Masahisa Watarai
|
タイトル |
Peculiar Paramecium hosts fail to establish a stable intracellular relationship with Legionella pneumophila
|
ジャーナル |
Frontiers in Microbiology
|
Abstract |
Legionella pneumophila, an intracellular human pathogen, establishes intracellular relationships with several protist hosts, including Paramecium caudatum. L. pneumophila can escape the normal digestion process and establish intracellular relationships in Paramecium. In this study, we identify new Paramecium strains that significantly reduce the number of L. pneumophila during infection. As a result, stable intracellular relationships between L. pneumophila and these Paramecium strains were not observed. These digestion-type Paramecium also showed high efficiency for Escherichia coli elimination compared to other strains of Paramecium. These results suggest that the digestion-type strains identified have high non-specific digestion activity. Although we evaluated the maturation process of Legionella-containing vacuoles (LCVs) in the Paramecium strains using LysoTracker, there were no discriminative changes in these LCVs compared to other Paramecium strains. Detailed understanding of the mechanisms of high digestion efficiency in these strains could be applied to water purification technologies and L. pneumophila elimination from environmental water.
|
公開日 |
2020-10-23
|
DOI |
10.3389/fmicb.2020.596731
|
IF |
4.236
|
リソース情報 |
ゾウリムシ |
PA000019A
PA040011A
PA040015A
PA041001A
PA042002A
PC000030A
PC000044A
PC000060A
PC000061A
PC000101A
PC000108A
PC000109A
PC000121A
PC000122A
PC000123A
PC000127A
PC000128A
PC000138A
PC000148A
PC011005A
PC011017A
PC011018A
PC012001A
PC012013A
PC031013A
PC031025A
PC032001A
PC032002A
PC032004A
PC041002A
PC042001A
PC042003A
PC051001A
PC061001A
PC061008A
PC061016A
PC062005A
PC062012A
PC071005A
PC072004A
PC120101A
PC120111A
PC121008A
PC121031A
PC121100A
PC122004A
PC122029A
PC122099A
PC130002A
PD000001A |