RRC ID 61095
著者 Kenta Watanabe, Yusei Higuchi, Mizuki Shimmura, Masato Tachibana, Masahiro Fujishima, Takashi Shimizu, Masahisa Watarai
タイトル Peculiar Paramecium hosts fail to establish a stable intracellular relationship with Legionella pneumophila
ジャーナル Frontiers in Microbiology
Abstract Legionella pneumophila, an intracellular human pathogen, establishes intracellular relationships with several protist hosts, including Paramecium caudatum. L. pneumophila can escape the normal digestion process and establish intracellular relationships in Paramecium. In this study, we identify new Paramecium strains that significantly reduce the number of L. pneumophila during infection. As a result, stable intracellular relationships between L. pneumophila and these Paramecium strains were not observed. These digestion-type Paramecium also showed high efficiency for Escherichia coli elimination compared to other strains of Paramecium. These results suggest that the digestion-type strains identified have high non-specific digestion activity. Although we evaluated the maturation process of Legionella-containing vacuoles (LCVs) in the Paramecium strains using LysoTracker, there were no discriminative changes in these LCVs compared to other Paramecium strains. Detailed understanding of the mechanisms of high digestion efficiency in these strains could be applied to water purification technologies and L. pneumophila elimination from environmental water.
公開日 2020-10-23
DOI 10.3389/fmicb.2020.596731
IF 4.236
リソース情報
ゾウリムシ PA000019A PA040011A PA040015A PA041001A PA042002A PC000030A PC000044A PC000060A PC000061A PC000101A PC000108A PC000109A PC000121A PC000122A PC000123A PC000127A PC000128A PC000138A PC000148A PC011005A PC011017A PC011018A PC012001A PC012013A PC031013A PC031025A PC032001A PC032002A PC032004A PC041002A PC042001A PC042003A PC051001A PC061001A PC061008A PC061016A PC062005A PC062012A PC071005A PC072004A PC120101A PC120111A PC121008A PC121031A PC121100A PC122004A PC122029A PC122099A PC130002A PD000001A