論文 - 詳細
| RRC ID | 61634 |
|---|---|
| 著者 | Nagao K, Shinohara N, Smit F, de Weijert M, Jannink S, Owada Y, Mulders P, Oosterwijk E, Matsuyama H. |
| タイトル | Fatty acid binding protein 7 may be a marker and therapeutic targets in clear cell renal cell carcinoma. |
| ジャーナル | BMC Cancer |
| Abstract |
BACKGROUND:To identify potential therapeutic target in clear cell renal cell carcinoma (ccRCC), we performed a transcriptome analysis. Our analysis showed that fatty acid binding protein 7 (FABP7) has the highest mean differential overexpression in ccRCC compared to normal kidney. We aimed to investigate the significance of FABP7 in ccRCC. METHODS:Immunohistochemical staining for 40 advanced ccRCC cases was performed to investigate correlation between clinicopathological parameters and FABP7. They were composed of 40-83 years old cases with 33 male, 22 cases with pT ≥ 3, 19 cases with M1, and 16 cases with grade 3. The effect of gene knockdown was analysed by a cell viability assay and invasion assay in FABP7-overexpressing cell lines (SKRC7 and SKRC10). RESULTS:Our immunohistochemical analysis showed that higher FABP7 expression significantly correlated with distant metastasis and poor cancer-specific survival (CSS; both p < 0.05). Functional suppression of FABP7 significantly inhibited SKRC10 cell growth (p < 0.05) and resulted in a significant reduction of the invasive potential (p < 0.01), but did not cause growth inhibition of SKRC7 cells. We found that The Cancer Genome Atlas Research Network (TCGA) database shows FABP6 and 7 as equally overexpressed in the FABP family. Functional suppression of fatty acid binding protein 6 (FABP6) resulted in significant growth inhibition of SKRC7 cells (p < 0.005). CONCLUSIONS:Functional suppression of FABP7 significantly reduced cell viability and invasive potential in a ccRCC cell line. FABP7 may play a role in progression in some metastatic ccRCCs. The suppressed function may be compensated by another FABP family member. |
| 巻・号 | 18(1) |
| ページ | 1114 |
| 公開日 | 2018-11-15 |
| DOI | 10.1186/s12885-018-5060-8 |
| PII | 10.1186/s12885-018-5060-8 |
| PMID | 30442117 |
| PMC | PMC6238291 |
| MeSH | Adult Aged Aged, 80 and over Antineoplastic Agents / pharmacology Antineoplastic Agents / therapeutic use Biomarkers, Tumor / antagonists & inhibitors Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Carcinoma, Renal Cell / drug therapy Carcinoma, Renal Cell / genetics Carcinoma, Renal Cell / mortality Carcinoma, Renal Cell / pathology* Cell Line, Tumor Cell Survival Disease Progression Fatty Acid-Binding Protein 7 / antagonists & inhibitors Fatty Acid-Binding Protein 7 / genetics Fatty Acid-Binding Protein 7 / metabolism* Fatty Acid-Binding Proteins / genetics Fatty Acid-Binding Proteins / metabolism* Female Gastrointestinal Hormones / genetics Gastrointestinal Hormones / metabolism* Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene Knockdown Techniques / methods Humans Kidney / pathology Kidney Neoplasms / drug therapy Kidney Neoplasms / genetics Kidney Neoplasms / mortality Kidney Neoplasms / pathology* Male Middle Aged Molecular Targeted Therapy / methods Neoplasm Invasiveness / pathology Progression-Free Survival RNA, Small Interfering / metabolism Survival Rate Tumor Suppressor Proteins / antagonists & inhibitors Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism* |
| IF | 3.15 |
| リソース情報 | |
| ヒト・動物細胞 | TUHR14TKB(RCB1383) |