論文 - 詳細
| RRC ID | 62161 |
|---|---|
| 著者 | Kamiyama F, Maeda T, Yamane T, Li YH, Ogukubo O, Otsuka T, Ueyama H, Takahashi S, Ohkubo I, Matsui N. |
| タイトル | Inhibition of vitronectin-mediated haptotaxis and haptoinvasion of MG-63 cells by domain 5 (D5(H)) of human high-molecular-weight kininogen and identification of a minimal amino acid sequence. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We found that human kinin-free high-molecular-weight kininogen (kf-HK) significantly inhibited vitronectin-mediated migration (haptotaxis) and invasive potentiation (haptoinvasion) of osteosarcoma (MG-63) cells but that HK, LK, the common heavy chain of HK and LK, and the light chain (D6(H)) of HK had no inhibitory effect. Recombinant GST-D5(H) (histidine-rich region of HK) obtained from Escherichia coli. (BL21) also inhibited both haptotaxis and haptoinvasion to about 30% of the control level in a dose-dependent manner. These findings suggest that a specific region of D5(H) is responsible for the inhibition of cell haptotaxis and haptoinvasion. Among the seven synthetic peptides covering D5(H), peptide H(479)KHGHGHGKHKNKGK(493) (P-5) inhibited both haptotaxis and haptoinvasion in a dose-dependent manner, suggesting that P-5 could possibly be utilized to prevent primary and secondary metastases of tumor cells. |
| 巻・号 | 288(4) |
| ページ | 975-80 |
| 公開日 | 2001-11-9 |
| DOI | 10.1006/bbrc.2001.5864 |
| PII | S0006-291X(01)95864-4 |
| PMID | 11689005 |
| MeSH | Amino Acid Sequence Cell Movement / drug effects* Collagen Dose-Response Relationship, Drug Drug Combinations Humans Kininogens / chemistry* Kininogens / pharmacology* Kininogens / therapeutic use Laminin Molecular Sequence Data Molecular Weight Neoplasm Invasiveness / pathology* Neoplasm Metastasis / drug therapy Neoplasm Metastasis / pathology* Osteosarcoma / drug therapy Osteosarcoma / pathology* Peptide Fragments / chemical synthesis Peptide Fragments / chemistry Peptide Fragments / pharmacology Peptide Fragments / therapeutic use Protein Structure, Tertiary Proteoglycans Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / pharmacology Recombinant Fusion Proteins / therapeutic use Tumor Cells, Cultured Vitronectin / antagonists & inhibitors* Vitronectin / pharmacology |
| IF | 2.985 |
| リソース情報 | |
| ヒト・動物細胞 | MG-63(RCB1890) |