| RRC ID |
63561
|
| Author |
Prümmer O, Cederblad B, Alm G, Drees N, Porzsolt F.
|
| Title |
Filter spot-ELISA for the enumeration of interferon-alpha antibody-secreting cells.
|
| Journal |
J Immunol Methods
|
| Abstract |
A filter spot-ELISA was developed for the enumeration of interferon-alpha (IFN-alpha) antibody secretion by murine and human cells. Various human IFN-alpha subtypes were coated onto nitrocellulose membranes by means of broad specificity bovine antibodies. Membranes were blocked with milk proteins, and antibodies released by individual cells during a 3 h culture period were visualized as distinct spots using peroxidase-conjugated, species-specific anti-immunoglobulin antibodies and diaminobenzidine/hydrogen peroxide substrate solution. The spot-ELISA is rapid, easy to perform, and highly sensitive. Possible applications include frequency estimates of IFN-alpha antibody-secreting cells in blood, tissues and hybridoma cultures as well as the evaluation of specificity and immunoglobulin class of the respective antibodies.
|
| Volume |
130(2)
|
| Pages |
187-93
|
| Published |
1990-7-3
|
| DOI |
10.1016/0022-1759(90)90047-y
|
| PII |
0022-1759(90)90047-Y
|
| PMID |
2373868
|
| IF |
1.913
|
| Resource |
| Human and Animal Cells |
MCF7(RCB1904) |
