RRC ID 63561
Author Prümmer O, Cederblad B, Alm G, Drees N, Porzsolt F.
Title Filter spot-ELISA for the enumeration of interferon-alpha antibody-secreting cells.
Journal J Immunol Methods
Abstract A filter spot-ELISA was developed for the enumeration of interferon-alpha (IFN-alpha) antibody secretion by murine and human cells. Various human IFN-alpha subtypes were coated onto nitrocellulose membranes by means of broad specificity bovine antibodies. Membranes were blocked with milk proteins, and antibodies released by individual cells during a 3 h culture period were visualized as distinct spots using peroxidase-conjugated, species-specific anti-immunoglobulin antibodies and diaminobenzidine/hydrogen peroxide substrate solution. The spot-ELISA is rapid, easy to perform, and highly sensitive. Possible applications include frequency estimates of IFN-alpha antibody-secreting cells in blood, tissues and hybridoma cultures as well as the evaluation of specificity and immunoglobulin class of the respective antibodies.
Volume 130(2)
Pages 187-93
Published 1990-7-3
DOI 10.1016/0022-1759(90)90047-y
PII 0022-1759(90)90047-Y
PMID 2373868
IF 1.913
Resource
Human and Animal Cells MCF7(RCB1904)