RRC ID 6444
著者 Xia D, Huang X, Zhang H.
タイトル The temporally regulated transcription factor sel-7 controls developmental timing in C. elegans.
ジャーナル Dev Biol
Abstract The temporal sequence of cell division and differentiation is explicitly controlled for succession and synchrony of developmental events. In this study we describe how the Caenorhabditis elegans gene sel-7 specifies the L3 stage-specific fate of seam cells, which adopt temporal specificities at each of four larval stages. Loss of function of sel-7 causes reiteration of the L2 stage fate at the L3 stage. sel-7 is involved in regulating the temporal expression pattern of hbl-1, which is a key factor in specifying the L2/L3 progression. We also show that sel-7 functions redundantly with other retarded heterochronic genes, including lin-46, daf-12 and the let-7 family miRNAs, in preventing adoption of the L2 fate at later stages. Expression of sel-7 in seam cells is temporally regulated through an evolutionarily conserved regulatory element located in intron 4 of sel-7. We further demonstrate that reiteration of the L2 proliferative seam cell division at the L3 stage in sel-7 mutants requires activity of the transcriptional mediator complex. Our study reveals that sel-7 functions as a novel heterochronic gene in controlling temporal cell identities and also demonstrates a role of the transcriptional mediator complex in integrating temporal information to specify seam cell division patterns in C. elegans.
巻・号 332(2)
ページ 246-57
公開日 2009-8-15
DOI 10.1016/j.ydbio.2009.05.574
PII S0012-1606(09)00904-X
PMID 19500563
MeSH Animals Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Division / physiology Cell Lineage Gene Expression Regulation, Developmental* Genes, Reporter Introns Mutation Nuclear Proteins RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism*
IF 2.896
引用数 4
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm2893