RRC ID 64545
Author Crane RA, Cardénas Valdez M, Castaneda N, Jackson CL, Riley CJ, Mostafa I, Kong W, Chhajed S, Chen S, Brusslan JA.
Title Negative Regulation of Age-Related Developmental Leaf Senescence by the IAOx Pathway, PEN1, and PEN3.
Journal Front Plant Sci
Abstract Early age-related developmental senescence was observed in Arabidopsis cyp79B2/cyp79B3 double mutants that cannot produce indole-3-acetaldoxime (IAOx), the precursor to indole glucosinolates (IGs), camalexin and auxin. The early senescence phenotype was not observed when senescence was induced by darkness. The cyp79B2/cyp79B3 mutants had lower auxin levels, but did not display auxin-deficient phenotypes. Camalexin biosynthesis mutants senesced normally; however, IG transport and exosome-related pen1/pen3 double mutants displayed early senescence. The early senescence in pen1/pen3 mutants depended on salicylic acid and was not observed in pen1 or pen3 single mutants. Quantitation of IGs showed reduced levels in cyp79B2/cyp79B3 mutants, but unchanged levels in pen1/pen3, even though both of these double mutants display early senescence. We discuss how these genetic data provide evidence that IAOx metabolites are playing a protective role in leaf senescence that is dependent on proper trafficking by PEN1 and PEN3, perhaps via the formation of exosomes.
Volume 10
Pages 1202
Published 2019-10-8
DOI 10.3389/fpls.2019.01202
PMID 31649689
IF 4.106
Resource
Arabidopsis / Cultured plant cells, genes pst04610 psh10821 psh19554