論文 - 詳細
| RRC ID | 64668 |
|---|---|
| 著者 | Nii T, Makino K, Tabata Y. |
| タイトル | A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system. |
| ジャーナル | Regen Ther |
| Abstract |
Introduction:The objective of this study is to design a cancer invasion model where the cancer invasion rate can be regulated in vitro. Methods:Cancer-associated fibroblasts (CAF) aggregates incorporating gelatin hydrogel microspheres (GM) containing various concentrations of transforming growth factor-β1 (TGF-β1) (CAF-GM-TGF-β1) were prepared. Alpha-smooth muscle actin (α-SMA) for the CAF aggregates was measured to investigate the CAF activation level by changing the concentration of TGF-β1. An invasion assay was performed to evaluate the cancer invasion rate by co-cultured of cancer cells with various CAF-GM-TGF-β1. Results:The expression level of α-SMA for CAF increased with an increased in the TGF-β1 concentration. When co-cultured with various types of CAF-GM-TGF-β1, the cancer invasion rate was well correlated with the α-SMA level. It is conceivable that the TGF-β1 concentration could modify the level of CAF activation, leading to the invasion rate of cancer cells. In addition, at the high concentrations of TGF-β1, the effect of a matrix metalloproteinase (MMP) inhibitor on the cancer invasion rate was observed. The higher invasion rate would be achieved through the higher MMP production. Conclusions:The present model is promising to realize the cancer invasion whose rate can be modified by changing the TGF-β1 concentration. |
| 巻・号 | 14 |
| ページ | 196-204 |
| 公開日 | 2020-6-1 |
| DOI | 10.1016/j.reth.2020.02.003 |
| PII | S2352-3204(20)30007-9 |
| PMID | 32154334 |
| PMC | PMC7058408 |
| IF | 2.286 |
| リソース情報 | |
| ヒト・動物細胞 | WA-hT(RCB2279) |