RRC ID 64713
Author Abe F, Ishida Y, Hisano H, Endo M, Komari T, Toki S, Sato K.
Title Protocol for Genome Editing to Produce Multiple Mutants in Wheat.
Journal STAR Protoc
Abstract Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for mutant production. The mutants produced by this protocol can be used for the analysis of gene function and crop improvement. For complete details on the use and execution of this protocol, please refer to Abe et al. (2019).
Volume 1(2)
Pages 100053
Published 2020-9-18
DOI 10.1016/j.xpro.2020.100053
PII S2666-1667(20)30040-X
PMID 33111098
PMC PMC7580089
Wheat Fielder